Inhibitors | Comment | Organism | Structure |
---|---|---|---|
epsilon-Acetyl-CoA | - |
Escherichia coli | |
additional information | partially inactivated by acyl-CoA substrates in absence of carboxylic acid substrates | Escherichia coli | |
p-chloromercuribenzoate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
acetyl-CoA | pH 8.1, 24°C | Escherichia coli | |
6.7 | - |
epsilon-Acetyl-CoA | pH 8.1, 24°C | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-associated | Escherichia coli | 16020 | - |
spheroplast | - |
Escherichia coli | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
2 * 23000 + 2 * 26000, 2 alpha and 2 beta subunits, SDS-PAGE | Escherichia coli |
26000 | - |
2 * 23000 + 2 * 26000, 2 alpha and 2 beta subunits, SDS-PAGE | Escherichia coli |
97800 | - |
sedimentation equilibrium centrifugation | Escherichia coli |
98000 | - |
SDS-PAGE | Escherichia coli |
99000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
butanoate + acetoacetyl-CoA | Escherichia coli | - |
butanoyl-CoA + acetoacetate | - |
r | |
butanoate + acetoacetyl-CoA | Escherichia coli C22 | - |
butanoyl-CoA + acetoacetate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
C22 | - |
Escherichia coli C22 | - |
C22 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | contains no detectable carbohydrate | Escherichia coli |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
117 | - |
fraction B | Escherichia coli |
160 | - |
fraction A | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-pentenoate + acetoacetyl-CoA | reaction at 21% the rate of acetate | Escherichia coli | 4-pentenoyl-CoA + acetoacetate | - |
r | |
acetate + acetoacetyl-CoA | best substrates | Escherichia coli | acetyl-CoA + acetoacetate | - |
r | |
acetate + acetoacetyl-CoA | best substrates | Escherichia coli C22 | acetyl-CoA + acetoacetate | - |
r | |
acetyl-CoA + acetoacetate | best substrates | Escherichia coli | acetate + acetoacetyl-CoA | - |
r | |
acetyl-CoA + acetoacetate | best substrates | Escherichia coli C22 | acetate + acetoacetyl-CoA | - |
r | |
butanoate + acetoacetyl-CoA | - |
Escherichia coli | butanoyl-CoA + acetoacetate | - |
r | |
butanoate + acetoacetyl-CoA | reaction at 68% the rate of acetate | Escherichia coli | butanoyl-CoA + acetoacetate | - |
r | |
butanoate + acetoacetyl-CoA | - |
Escherichia coli C22 | butanoyl-CoA + acetoacetate | - |
r | |
butanoate + acetoacetyl-CoA | reaction at 68% the rate of acetate | Escherichia coli C22 | butanoyl-CoA + acetoacetate | - |
r | |
butyrate + acetyl-CoA | reaction at 56% the rate of acetoacetate, reverse reaction at 86% the rate of acetoacetyl-CoA | Escherichia coli | butyryl-CoA + acetate | - |
r | |
crotonyl-CoA + acetate | reaction at 4% the rate of acetoacetylCoA | Escherichia coli | crotonate + acetyl-CoA | - |
r | |
DL-3-hydroxybutyrate + acetyl-CoA | reaction at 9% the rate of acetoacetate | Escherichia coli | 3-hydroxybutyryl-CoA + acetate | - |
r | |
epsilon-acetyl-CoA + acetoacetate | reaction at 1% the rate of acetylCoA | Escherichia coli | acetate + acetoacetyl-CoA | - |
r | |
formate + acetoacetyl-CoA | reaction at 7% the rate of acetate | Escherichia coli | formyl-CoA + acetoacetate | - |
r | |
additional information | decanoyl-CoA and succinyl-CoA cannot replace acetoacetyl-CoA, succinate, malonate and 2,3-butandienoate cannot replace acetate, 3-carbon dicarboxylic and 4-carbon dicarboxylic acids are completely inactive as substrates | Escherichia coli | ? | - |
? | |
additional information | decanoyl-CoA and succinyl-CoA cannot replace acetoacetyl-CoA, succinate, malonate and 2,3-butandienoate cannot replace acetate, 3-carbon dicarboxylic and 4-carbon dicarboxylic acids are completely inactive as substrates | Escherichia coli C22 | ? | - |
? | |
octanoate + acetoacetyl-CoA | reaction at 3% the rate of acetate | Escherichia coli | octanoyl-CoA + acetoacetate | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 23000 + 2 * 26000, 2 alpha and 2 beta subunits, SDS-PAGE | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
10.6 | - |
epsilon-Acetyl-CoA | pH 8.1, 24°C | Escherichia coli |