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Literature summary for 2.8.3.8 extracted from

  • Mullins, E.A.; Kappock, T.J.
    Crystal structures of Acetobacter aceti succinyl-coenzyme A (CoA):acetate CoA-transferase reveal specificity determinants and illustrate the mechanism used by class I CoA-transferases (2012), Biochemistry, 51, 8422-8434.
    View publication on PubMed
Search for more literature for 2.8.3.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41(DE3) cells Acetobacter aceti

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 1.7-2.0 M ammonium sulfate, 0.2 M sodium chloride, 0.1 M sodium cacodylate (pH 6.5), and 25 mM 2-mercaptoethanol Acetobacter aceti

Protein Variants

Protein Variants Comment Organism
E294A the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Acetobacter aceti
E435A insoluble mutant Acetobacter aceti
E435D the mutant shows almost wild type activity Acetobacter aceti
E435Q insoluble mutant Acetobacter aceti
R228E the mutant has a specific defect in its ability to bind both carboxylate substrates succinyl-CoA and acetyl-CoA and does not use 4-aminobutyrate as substrate Acetobacter aceti
S71A the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Acetobacter aceti

Inhibitors

Inhibitors Comment Organism Structure
acetate
-
 Acetobacter aceti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
110
-
 acetate wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti

Organism

Organism UniProt Comment Textmining
Acetobacter aceti B3EY95
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, dye-affinity chromatography, ion-exchange column chromatography, and gel filtration Acetobacter aceti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetate + succinyl-CoA 5.1% activity compared to succinate Acetobacter aceti succinate + acetyl-CoA
-
 r
acetate + succinyl-CoA 5.1% activity compared to succinate Acetobacter aceti 1023 succinate + acetyl-CoA
-
 r
additional information less than 1% activity with acetoacetate, propionate, D-malate, fumarate, L-malate, formate, oxaloacetate, DL-methylsuccinate, and glutarate. No activity is detected with dethiaacetyl-CoA, glycolate, glyoxylate, oxalate, trifluoroacetate, DL-lactate, L-lactate, malonate, pyruvate, maleate, butyrate, D-tartrate, L-tartrate, alpha-ketoglutarate, citrate, or DL-isocitrate Acetobacter aceti ?
-
 ?
additional information less than 1% activity with acetoacetate, propionate, D-malate, fumarate, L-malate, formate, oxaloacetate, DL-methylsuccinate, and glutarate. No activity is detected with dethiaacetyl-CoA, glycolate, glyoxylate, oxalate, trifluoroacetate, DL-lactate, L-lactate, malonate, pyruvate, maleate, butyrate, D-tartrate, L-tartrate, alpha-ketoglutarate, citrate, or DL-isocitrate Acetobacter aceti 1023 ?
-
 ?
succinate + acetyl-CoA 100% activity Acetobacter aceti acetate + succinyl-CoA
-
 r
succinate + acetyl-CoA 100% activity Acetobacter aceti 1023 acetate + succinyl-CoA
-
 r

Synonyms

Synonyms Comment Organism
AarC
-
 Acetobacter aceti
SCACT
-
 Acetobacter aceti
succinyl-CoA:acetate CoA-transferase
-
 Acetobacter aceti
succinyl-coenzyme A:acetate CoA-transferase
-
 Acetobacter aceti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
290
-
 acetate wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1600
-
 acetate wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti

General Information

General Information Comment Organism
metabolism succinyl-CoA:acetate CoA-transferase is an acetic acid resistance factor (AarC) with a role in a variant citric acid cycle Acetobacter aceti
physiological function the enzyme is an acetic acid resistance factor that is required for acetate resistance Acetobacter aceti

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.029
-
 acetate mutant enzyme S71A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti
0.03
-
 succinate mutant enzyme S71A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti
0.074
-
 succinyl-CoA mutant enzyme S71A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti
0.13
-
 succinyl-CoA mutant enzyme R228E, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti
0.16
-
 acetate mutant enzyme N347A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti
0.3
-
 succinate mutant enzyme R228E, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti
0.39
-
 succinyl-CoA mutant enzyme N347A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti
0.6
-
 acetate mutant enzyme R228E, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti
0.7
-
 acetyl-CoA mutant enzyme N347A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti
0.7
-
 acetyl-CoA mutant enzyme S71A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti
2.3
-
 acetyl-CoA mutant enzyme R228E, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti
2.7
-
 succinate mutant enzyme N347A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti
3
-
 acetate mutant enzyme E435D, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti
3.4
-
 acetyl-CoA wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti
9
-
 succinyl-CoA mutant enzyme E435D, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti
9
-
 succinyl-CoA wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti
52
-
 acetyl-CoA mutant enzyme E435D, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti
73
-
 succinate mutant enzyme E435D, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti
79
-
 succinate wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti
2600
-
 acetate wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C Acetobacter aceti