Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C41(DE3) cells | Acetobacter aceti |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 1.7-2.0 M ammonium sulfate, 0.2 M sodium chloride, 0.1 M sodium cacodylate (pH 6.5), and 25 mM 2-mercaptoethanol | Acetobacter aceti |
Protein Variants | Comment | Organism |
---|---|---|
E294A | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Acetobacter aceti |
E435A | insoluble mutant | Acetobacter aceti |
E435D | the mutant shows almost wild type activity | Acetobacter aceti |
E435Q | insoluble mutant | Acetobacter aceti |
R228E | the mutant has a specific defect in its ability to bind both carboxylate substrates succinyl-CoA and acetyl-CoA and does not use 4-aminobutyrate as substrate | Acetobacter aceti |
S71A | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Acetobacter aceti |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetate | - |
Acetobacter aceti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
110 | - |
acetate | wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetobacter aceti | B3EY95 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, dye-affinity chromatography, ion-exchange column chromatography, and gel filtration | Acetobacter aceti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetate + succinyl-CoA | 5.1% activity compared to succinate | Acetobacter aceti | succinate + acetyl-CoA | - |
r | |
acetate + succinyl-CoA | 5.1% activity compared to succinate | Acetobacter aceti 1023 | succinate + acetyl-CoA | - |
r | |
additional information | less than 1% activity with acetoacetate, propionate, D-malate, fumarate, L-malate, formate, oxaloacetate, DL-methylsuccinate, and glutarate. No activity is detected with dethiaacetyl-CoA, glycolate, glyoxylate, oxalate, trifluoroacetate, DL-lactate, L-lactate, malonate, pyruvate, maleate, butyrate, D-tartrate, L-tartrate, alpha-ketoglutarate, citrate, or DL-isocitrate | Acetobacter aceti | ? | - |
? | |
additional information | less than 1% activity with acetoacetate, propionate, D-malate, fumarate, L-malate, formate, oxaloacetate, DL-methylsuccinate, and glutarate. No activity is detected with dethiaacetyl-CoA, glycolate, glyoxylate, oxalate, trifluoroacetate, DL-lactate, L-lactate, malonate, pyruvate, maleate, butyrate, D-tartrate, L-tartrate, alpha-ketoglutarate, citrate, or DL-isocitrate | Acetobacter aceti 1023 | ? | - |
? | |
succinate + acetyl-CoA | 100% activity | Acetobacter aceti | acetate + succinyl-CoA | - |
r | |
succinate + acetyl-CoA | 100% activity | Acetobacter aceti 1023 | acetate + succinyl-CoA | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AarC | - |
Acetobacter aceti |
SCACT | - |
Acetobacter aceti |
succinyl-CoA:acetate CoA-transferase | - |
Acetobacter aceti |
succinyl-coenzyme A:acetate CoA-transferase | - |
Acetobacter aceti |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
290 | - |
acetate | wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1600 | - |
acetate | wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti |
General Information | Comment | Organism |
---|---|---|
metabolism | succinyl-CoA:acetate CoA-transferase is an acetic acid resistance factor (AarC) with a role in a variant citric acid cycle | Acetobacter aceti |
physiological function | the enzyme is an acetic acid resistance factor that is required for acetate resistance | Acetobacter aceti |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
acetate | mutant enzyme S71A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti | |
0.03 | - |
succinate | mutant enzyme S71A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti | |
0.074 | - |
succinyl-CoA | mutant enzyme S71A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti | |
0.13 | - |
succinyl-CoA | mutant enzyme R228E, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti | |
0.16 | - |
acetate | mutant enzyme N347A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti | |
0.3 | - |
succinate | mutant enzyme R228E, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti | |
0.39 | - |
succinyl-CoA | mutant enzyme N347A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti | |
0.6 | - |
acetate | mutant enzyme R228E, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti | |
0.7 | - |
acetyl-CoA | mutant enzyme N347A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti | |
0.7 | - |
acetyl-CoA | mutant enzyme S71A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti | |
2.3 | - |
acetyl-CoA | mutant enzyme R228E, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti | |
2.7 | - |
succinate | mutant enzyme N347A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti | |
3 | - |
acetate | mutant enzyme E435D, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti | |
3.4 | - |
acetyl-CoA | wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti | |
9 | - |
succinyl-CoA | mutant enzyme E435D, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti | |
9 | - |
succinyl-CoA | wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti | |
52 | - |
acetyl-CoA | mutant enzyme E435D, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti | |
73 | - |
succinate | mutant enzyme E435D, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti | |
79 | - |
succinate | wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti | |
2600 | - |
acetate | wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C | Acetobacter aceti |