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Literature summary for 2.8.3.5 extracted from

  • Bregere, C.; Rebrin, I.; Gallaher, T.K.; Sohal, R.S.
    Effects of age and calorie restriction on tryptophan nitration, protein content, and activity of succinyl-CoA:3-ketoacid CoA transferase in rat kidney mitochondria (2009), Free Radic. Biol. Med., 48, 609-618.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.3.5

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 2 * 58000, SDS-PAGE Rattus norvegicus
58000
-
 2 * 58000, full-length protein, SDS-PAGE Rattus norvegicus
120000
-
 gel filtration Rattus norvegicus
120000
-
 MALDI-TOF mass spectrometry Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinyl-CoA + acetoacetate Rattus norvegicus
-
 succinate + acetoacetyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 male Fischer 344 rats
-
Rattus norvegicus B2GV06
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information the enzyme is a target for nitration, tryptophan 372, rather than a tyrosine residue, is the actual site of simultaneous additions of nitro and hydroxy groups, impact on enzyme activity, mass spectrometric analysis, overview Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
BioSep-SEC-S 3000 gel filtration Rattus norvegicus
native enzyme from mitochondria by chromatofocusing and gel filtration to over 75% purity Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Rattus norvegicus
-
kidney age-related decreases in SCOT protein amount and catalytic activity: between 4 and 24 months of age, the amounts of SCOT protein and catalytic activity decrease by 55 and 45%, respectively Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-CoA + acetoacetate
-
 Rattus norvegicus succinate + acetoacetyl-CoA
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 58000, SDS-PAGE Rattus norvegicus
homodimer 2 * 58000, full-length protein, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
SCOT
-
 Rattus norvegicus
succinyl-CoA:3-ketoacid CoA transferase
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
succinyl-CoA
-
 Rattus norvegicus

pI Value

Organism Comment pI Value Maximum pI Value
Rattus norvegicus isoelectric focusing
-
 7

Expression

Organism Comment Expression
Rattus norvegicus between 4 and 24 months of age, the amount of the enzyme protein and catalytic activity decrease by 55% and 45%, respectively down

General Information

General Information Comment Organism
malfunction loss of succinyl-CoA:3-ketoacid CoA transferase protein in aged rats may attenuate the capacity of kidney mitochondria to utilize ketone bodies for energy production Rattus norvegicus
metabolism SCOT is a rate-limiting enzyme in the degradation of ketone bodies Rattus norvegicus
physiological function loss of SCOT protein in the aged rats may attenuate the capacity of kidney mitochondria to utilize ketone bodies for energy production Rattus norvegicus