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Literature summary for 2.8.3.5 extracted from
- Purification and properties of succinyl-coenzyme A-3-oxo acid coenzyme A-transferase from sheep kidney (1978), Biochem. J., 173, 759-765.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition | Sus scrofa |
| additional information | - |
amino acid composition | Ovis aries |
| 56000 | - |
2 * 56000, SDS-PAGE | Ovis aries |
| 102000 | - |
analytical ultracentrifugation | Ovis aries |
| 110000 | - |
gel filtration | Ovis aries |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ovis aries | - |
- |
- |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ovis aries |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Sus scrofa | - |
| brain | - |
Ovis aries | - |
| heart | - |
Sus scrofa | - |
| heart | - |
Ovis aries | - |
| kidney | - |
Sus scrofa | - |
| kidney | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 200 | - |
- |
Ovis aries |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, at least 1 month | Sus scrofa |
| -20°C, at least 1 month | Ovis aries |
| storage as ammonium sulfate suspension leads to rapid loss of activity | Ovis aries |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinyl-CoA + acetoacetate | - |
Sus scrofa | succinate + acetoacetyl-CoA | - |
r |  |
| succinyl-CoA + acetoacetate | - |
Ovis aries | succinate + acetoacetyl-CoA | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Sus scrofa |
| dimer | 2 * 56000, SDS-PAGE | Ovis aries |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Sus scrofa |
| 30 | - |
assay at | Ovis aries |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Sus scrofa | pH gradient pH 3.5-10 or pH 7-10 in glycerol gradient, brain enzyme | - |
4.8 |
| Sus scrofa | pH gradient pH 3.5-10 or pH 7-10 in glycerol gradient, heart and kidney enzyme | - |
5.5 |
| Ovis aries | pH gradient pH 3.5-10 or pH 7-10 in glycerol gradient, brain enzyme | 6.8 | 6.3 |
| Ovis aries | pH gradient pH 3.5-10 or pH 7-10 in glycerol gradient, heart enzyme | - |
8.2 |
| Ovis aries | pH gradient pH 3.5-10 or pH 7-10 in glycerol gradient, purified kidney enzyme | - |
8.4 |
| Ovis aries | pH gradient pH 3.5-10 or pH 7-10 in glycerol gradient, purified kidney enzyme | - |
8.7 |
| Ovis aries | pH gradient pH 3.5-10 or pH 7-10 in glycerol gradient, enzyme in kidney extract | - |
9 |
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