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Literature summary for 2.8.3.5 extracted from

  • Hersh, L.B.; Jencks, W.P.
    Coenzyme A transferase. Kinetics and exchange reactions (1967), J. Biol. Chem., 242, 3468-3480.
No PubMed abstract available
Search for more literature for 2.8.3.5

Inhibitors

Inhibitors Comment Organism Structure
2,4-Dinitrophenylacetate at pH 7.9, less inactivating activity at pH 7, acetoacetyl-CoA protects Sus scrofa
4-nitrophenylacetate at pH 7.9, less inactivating activity at pH 7 Sus scrofa
Acetic anhydride
-
 Sus scrofa
acetoacetate kinetics; product inhibition Sus scrofa
acetoacetyl-CoA EDTA, trisodium citrate and diphosphate protect, too, addition of Cu2+, Mn2+, Ca2+ or Zn2+ (decreasing order) restores inactivating activity of acetoacetyl-CoA; in the absence of succinate, cysteine restores, succinate or 0.1 M NaCl protects Sus scrofa
Acetylimidazole equally efficient at pH 7 and 7.9 Sus scrofa
citrate 0.167 M, weak Sus scrofa
Glutarate 0.1 M, weak Sus scrofa
HPO42- 0.1 M, weak Sus scrofa
malate 0.1 M, weak Sus scrofa
malonate 0.1 M, weak Sus scrofa
Monovalent anions decreasing order of effectiveness: SCN-, ClO4-, I-, Br-, Cl-, not F- Sus scrofa
NaCl kinetics, 24% inhibition at 10 mM Sus scrofa
NaI 57% inhibition at 10 mM Sus scrofa
SCN- 47% inhibition at 10 mM Sus scrofa
SO42- 0.1 M, weak Sus scrofa
succinate kinetics; product inhibition Sus scrofa
succinyl-CoA
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic study Sus scrofa
0.2
-
 acetoacetate cosusbstrate: succinyl-CoA, pH 8.1 Sus scrofa
0.72
-
 acetoacetyl-CoA cosusbstrate: succinate, pH 8.1 Sus scrofa
4.2
-
 succinyl-CoA cosusbstrate: acetoacetate, pH 8.1 Sus scrofa
36
-
 succinate cosusbstrate: acetoacetyl-CoA, pH 8.1 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
 gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Sus scrofa

Reaction

Reaction Comment Organism Reaction ID
succinyl-CoA + a 3-oxo acid = succinate + a 3-oxoacyl-CoA mechanism Sus scrofa
a

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3
-
-
 Sus scrofa

Storage Stability

Storage Stability Organism
-20°C, 1.4 mg protein/ml, 0.02 M potassium phosphate buffer, pH 7.4, t1/2: 9 months Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information catalyzes exchange reactions in the absence of cosubstrates: succinate/succinyl-CoA and acetoacetate/acetoacetyl-CoA Sus scrofa ?
-
 ?
succinyl-CoA + acetoacetate
-
 Sus scrofa succinate + acetoacetyl-CoA
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 1 min, inactivation at pH-values below pH 3.1 or in 0.1 M NaOH, slow loss of activity at pH 5 and pH 10.7 Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
37.3
-
 succinyl-CoA pH 8.1 Sus scrofa
933
-
 acetoacetyl-CoA pH 8.1 Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.7
-
 Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
7.1 8.7 about 50% of activity at pH 7.1, optimum at pH 8-8.7 Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.1
-
 below, 1 min at 25°C, inactivation Sus scrofa
5
-
 slow loss of activity at 25°C Sus scrofa
10.7
-
 slow loss of activity at 25°C Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.13
-
 acetoacetyl CoA pH 8.1 Sus scrofa
0.78
-
 acetoacetate pH 8.1 Sus scrofa
1
-
 succinate pH 8.1 Sus scrofa
1.9
-
 succinyl-CoA pH 8.1 Sus scrofa