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Literature summary for 2.8.3.28 extracted from

  • Schuehle, K.; Nies, J.; Heider, J.
    An indoleacetate-CoA ligase and a phenylsuccinyl-CoA transferase involved in anaerobic metabolism of auxin (2016), Environ. Microbiol., 18, 3120-3132 .
    View publication on PubMed
Search for more literature for 2.8.3.28

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Aromatoleum aromaticum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
 (R/S)-phenylsuccinyl-CoA at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
0.064
-
 (S)-benzylsuccinate at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
0.078
-
 (R/S)-benzylsuccinate at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
0.082
-
 (R/S)-benzylsuccinyl-CoA at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
0.092
-
 (R)-benzylsuccinate at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
0.103
-
 2-oxoindole-3-acetate at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
0.142
-
 indole-3-acetate at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
0.178
-
 (R/S)-phenylsuccinate at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 2 mM used in assay conditions Aromatoleum aromaticum

Organism

Organism UniProt Comment Textmining
Aromatoleum aromaticum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
strep-tactin affinity column chromatography Aromatoleum aromaticum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.024
-
 with (R/S)-benzylsuccinate plus (R/S)-phenylsuccinyl-CoA as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
0.085
-
 with (S)-benzylsuccinate plus succinyl-CoA as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
0.103
-
 with (R/S)-benzylsuccinate plus succinyl-CoA as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
0.108
-
 with (R/S)-phenylsuccinate plus (R/S)-benzylsuccinyl-CoA as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
0.122
-
 with (R)-benzylsuccinate plus succinyl-CoA as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
0.136
-
 with 2-oxoindole-3-acetate plus succinyl-CoA as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
0.188
-
 with indole-3-acetate plus succinyl-CoA as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
0.235
-
 with (R/S)-phenylsuccinate plus succinyl-CoA as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-benzylsuccinate + succinyl-CoA 52% activity compared to (R/S)-phenylsuccinate Aromatoleum aromaticum (R)-2-benzylsuccinyl-CoA + succinate
-
 ?
(R/S)-benzylsuccinate + (R/S)-phenylsuccinyl-CoA 10% activity compared to (R/S)-phenylsuccinate plus succinyl-CoA Aromatoleum aromaticum (R/S)-benzylsuccinyl-CoA + (R/S)-phenylsuccinate
-
 ?
(R/S)-benzylsuccinate + succinyl-CoA 44% activity compared to (R/S)-phenylsuccinate Aromatoleum aromaticum (R/S)-2-benzylsuccinyl-CoA + succinate
-
 ?
(R/S)-phenylsuccinate + (R/S)-benzylsuccinyl-CoA 46% activity compared to (R/S)-phenylsuccinate plus succinyl-CoA Aromatoleum aromaticum (R/S)-phenylsuccinyl-CoA + (R/S)-benzylsuccinate
-
 ?
(R/S)-phenylsuccinate + succinyl-CoA 100% activity, (R/S)-phenylsuccinate is converted to an equal mixture of both regioisomers (2- and 3-phenylsuccinyl-CoA) Aromatoleum aromaticum (R/S)-2-phenylsuccinyl-CoA + (R/S)-3-phenylsuccinyl-CoA + succinate
-
 ?
(S)-benzylsuccinate + succinyl-CoA 36% activity compared to (R/S)-phenylsuccinate Aromatoleum aromaticum (S)-2-benzylsuccinyl-CoA + succinate
-
 ?
2'-(2-aminophenyl)succinate + succinyl-CoA
-
 Aromatoleum aromaticum 3'-(2-aminophenyl)succinyl-CoA + succinate
-
 ?
2-oxoindole-3-acetate + succinyl-CoA 58% activity compared to (R/S)-phenylsuccinate Aromatoleum aromaticum 2-oxoindole-3-acetyl-CoA + succinate
-
 ?
indole-3-acetate + succinyl-CoA 80% activity compared to (R/S)-phenylsuccinate Aromatoleum aromaticum indole-3-acetyl-CoA + succinate
-
 ?
additional information no activity with acetyl-CoA, benzylmalonate, and cinnamate Aromatoleum aromaticum ?
-
-

Subunits

Subunits Comment Organism
? x * 40000, Strep-tagged enzyme, SDS-PAGE Aromatoleum aromaticum
? x * 45000, Strep-tagged enzyme, calculated from amino acid sequence Aromatoleum aromaticum

Synonyms

Synonyms Comment Organism
IaaL
-
 Aromatoleum aromaticum