BRENDA - Enzyme Database
show all sequences of 2.8.3.18

Functional dissection of the bipartite active site of the class I coenzyme A (CoA)-transferase succinyl-CoA:acetate CoA-transferase

Murphy, J.; Mullins, E.; Kappock, T.; Front. Chem. 4, 23 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli C41(DE3) cells
Acetobacter aceti
Crystallization (Commentary)
Crystallization
Organism
enzyme bound to dethiaacetyl-CoA and acetate, hanging drop vapor diffusion method, using 0.9 M sodium citrate, 0.1 M imidazole-HCl, pH 8.2, and 25 mM 2-mercaptoethanol
Acetobacter aceti
Engineering
Amino acid exchange
Commentary
Organism
N347A
the mutant retains about 15% of wild type specific activity
Acetobacter aceti
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
succinyl-CoA + acetate
Acetobacter aceti
-
acetyl-CoA + succinate
-
-
?
succinyl-CoA + acetate
Acetobacter aceti 1023
-
acetyl-CoA + succinate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acetobacter aceti
B3EY95
-
-
Acetobacter aceti 1023
B3EY95
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
succinyl-CoA + acetate
-
738287
Acetobacter aceti
acetyl-CoA + succinate
-
-
-
?
succinyl-CoA + acetate
-
738287
Acetobacter aceti 1023
acetyl-CoA + succinate
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli C41(DE3) cells
Acetobacter aceti
Crystallization (Commentary) (protein specific)
Crystallization
Organism
enzyme bound to dethiaacetyl-CoA and acetate, hanging drop vapor diffusion method, using 0.9 M sodium citrate, 0.1 M imidazole-HCl, pH 8.2, and 25 mM 2-mercaptoethanol
Acetobacter aceti
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
N347A
the mutant retains about 15% of wild type specific activity
Acetobacter aceti
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
succinyl-CoA + acetate
Acetobacter aceti
-
acetyl-CoA + succinate
-
-
?
succinyl-CoA + acetate
Acetobacter aceti 1023
-
acetyl-CoA + succinate
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
succinyl-CoA + acetate
-
738287
Acetobacter aceti
acetyl-CoA + succinate
-
-
-
?
succinyl-CoA + acetate
-
738287
Acetobacter aceti 1023
acetyl-CoA + succinate
-
-
-
?
Other publictions for EC 2.8.3.18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738287
Murphy
Functional dissection of the b ...
Acetobacter aceti 1023, Acetobacter aceti
Front. Chem.
4
23
2016
-
-
1
1
1
-
-
-
-
-
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2
-
4
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2
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1
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1
1
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2
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2
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-
721675
Mullins
Crystal structures of Acetobac ...
Acetobacter aceti 1023, Acetobacter aceti
Biochemistry
51
8422-8434
2012
-
-
1
1
7
-
3
1
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-
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1
-
4
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-
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1
-
-
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20
-
1
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1
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2
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3
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1
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1
7
-
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3
3
1
-
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1
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20
-
1
-
-
-
1
-
2
-
-
3
3
-
25
25
692865
Mullins
A specialized citric acid cycl ...
Acetobacter aceti
J. Bacteriol.
190
4933-4940
2008
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-
1
-
-
-
3
8
-
-
2
-
-
2
-
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1
1
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4
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6
1
1
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7
1
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5
1
-
-
-
2
-
-
-
-
-
5
8
12
-
-
2
-
-
-
-
1
-
-
4
-
6
1
1
-
-
12
1
-
-
1
-
1
1
-
5
5
693049
van Grinsven
Acetate:succinate CoA-transfer ...
Trichomonas vaginalis
J. Biol. Chem.
283
1411-1418
2008
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-
1
-
-
-
-
1
1
-
1
-
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6
-
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1
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1
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1
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1
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1
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1
1
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1
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1
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1
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1
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1
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662286
Riviere
Acetyl:succinate CoA-transfera ...
Trypanosoma brucei
J. Biol. Chem.
279
45337-45346
2004
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1
1
-
-
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1
1
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2
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1
3
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1
1
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1
1
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1
3
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2
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723135
Steinbüchel
Anaerobic pyruvate metabolism ...
Tritrichomonas foetus, Tritrichomonas foetus ATCC 30924
Mol. Biochem. Parasitol.
20
57-65
1986
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1
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1
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