BRENDA - Enzyme Database
show all sequences of 2.8.3.16

Stable expression of the oxc and frc genes from Oxalobacter formigenes in human embryo kidney 293 cells: implications for gene therapy of hyperoxaluria

Ye, Z.Q.; Kong, D.B.; Chen, Z.Q.; Yao, L.F.; Guo, H.; Yu, X.; Liu, G.L.; Yang, W.M.; Int. J. Mol. Med. 20, 521-526 (2007)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
gene therapy of hyperoxaluria
Oxalobacter formigenes
Cloned(Commentary)
Commentary
Organism
stable expression in human embryo kidney 293 cells
Oxalobacter formigenes
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
of transfected HEK-293 cells
Oxalobacter formigenes
5737
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
formyl-CoA + oxalate
Oxalobacter formigenes
oxalyl-CoA decarboxylase and formyl-CoA transferase are the key enzymes in the oxalate catabolism of Oxalobacter formigenes which dwell in the intestine of vertebrates and have an important symbiotic relationship with their hosts
formate + oxalyl-CoA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oxalobacter formigenes
-
present in the intestine of Homo sapiens, the strain was isolated from fecal samples of healthy Chinese
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
formyl-CoA + oxalate
-
692677
Oxalobacter formigenes
formate + oxalyl-CoA
-
-
-
?
formyl-CoA + oxalate
oxalyl-CoA decarboxylase and formyl-CoA transferase are the key enzymes in the oxalate catabolism of Oxalobacter formigenes which dwell in the intestine of vertebrates and have an important symbiotic relationship with their hosts
692677
Oxalobacter formigenes
formate + oxalyl-CoA
-
-
-
?
Application (protein specific)
Application
Commentary
Organism
medicine
gene therapy of hyperoxaluria
Oxalobacter formigenes
Cloned(Commentary) (protein specific)
Commentary
Organism
stable expression in human embryo kidney 293 cells
Oxalobacter formigenes
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
of transfected HEK-293 cells
Oxalobacter formigenes
5737
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
formyl-CoA + oxalate
Oxalobacter formigenes
oxalyl-CoA decarboxylase and formyl-CoA transferase are the key enzymes in the oxalate catabolism of Oxalobacter formigenes which dwell in the intestine of vertebrates and have an important symbiotic relationship with their hosts
formate + oxalyl-CoA
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
formyl-CoA + oxalate
-
692677
Oxalobacter formigenes
formate + oxalyl-CoA
-
-
-
?
formyl-CoA + oxalate
oxalyl-CoA decarboxylase and formyl-CoA transferase are the key enzymes in the oxalate catabolism of Oxalobacter formigenes which dwell in the intestine of vertebrates and have an important symbiotic relationship with their hosts
692677
Oxalobacter formigenes
formate + oxalyl-CoA
-
-
-
?
Other publictions for EC 2.8.3.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739759
Herve
Diversity and ecology of oxalo ...
Actinobacteria, Firmicutes, Proteobacteria
World J. Microbiol. Biotechnol.
32
28
2016
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3
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3
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3
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3
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-
726452
Mullins
Formyl-coenzyme A (CoA):oxalat ...
Acetobacter aceti 1023, Acetobacter aceti
Protein Sci.
21
686-696
2012
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-
1
1
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2
1
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4
2
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4
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1
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2
1
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2
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1
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1
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2
1
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4
2
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1
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2
1
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2
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1
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1
2
2
692851
Toyota
Differential substrate specifi ...
Escherichia coli, Escherichia coli MG1655, Oxalobacter formigenes
J. Bacteriol.
190
2556-2564
2008
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2
1
2
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4
10
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3
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31
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2
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6
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2
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2
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1
2
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4
2
10
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3
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2
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6
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693216
Berthold
Reinvestigation of the catalyt ...
Oxalobacter formigenes
J. Biol. Chem.
283
6519-6529
2008
-
-
1
1
3
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2
8
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1
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2
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1
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1
2
1
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2
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1
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1
3
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2
2
8
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1
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1
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1
2
1
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693608
Khammar
Use of the frc gene as a molec ...
Ancylobacter oerskovii, Ancylobacter polymorphus, Arquibacter sp., Azorhizobium sp., Azospirillum brasilense, Azospirillum lipoferum, Bradyrhizobium japonicum, Bradyrhizobium sp., Cupriavidus necator, Cupriavidus necator JMP 134-1, Cupriavidus oxalaticus, Escherichia coli, Herminiimonas arsenicoxydans, Herminiimonas saxobsidens, Janthinobacterium sp. Marseille, Methylobacterium organophilum, Methylorubrum extorquens, Methylorubrum thiocyanatum, Oxalicibacterium flavum, Oxalobacter formigenes, Pandoraea sp., Paraburkholderia xenovorans, Rhodopseudomonas palustris, Shigella flexneri, Starkeya novella, Streptomyces avermitilis, Streptomyces coelicolor, Streptomyces violaceoruber, Variovorax paradoxus, Xanthobacter autotrophicus, Xanthobacter flavus, Xanthomonas sp.
J. Microbiol. Methods
76
120-127
2008
-
93
31
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36
-
39
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62
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72
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105
35
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36
-
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70
-
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72
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674225
Turroni
Oxalate consumption by lactoba ...
Lactobacillus acidophilus
J. Appl. Microbiol.
103
1600-1609
2007
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-
1
-
-
-
-
-
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1
1
-
5
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2
1
1
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1
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1
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1
1
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2
1
1
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1
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692373
Lewanika
Lactobacillus gasseri Gasser A ...
Lactobacillus gasseri, Lactobacillus gasseri Gasser AM63T
FEMS Microbiol. Ecol.
61
110-120
2007
-
1
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2
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6
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4
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1
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2
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4
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692677
Ye
Stable expression of the oxc a ...
Oxalobacter formigenes
Int. J. Mol. Med.
20
521-526
2007
-
1
1
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1
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1
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4
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2
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1
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1
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2
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-
671452
Azcarate-Peril
Transcriptional and functional ...
Bifidobacterium animalis subsp. lactis, Lactobacillus acidophilus, Lactobacillus acidophilus NCFM, Lactobacillus gasseri
Appl. Environ. Microbiol.
72
1891-1899
2006
1
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3
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1
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2
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14
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4
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3
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1
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4
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662253
Jonsson
Kinetic and mechanistic charac ...
Oxalobacter formigenes
J. Biol. Chem.
279
36003-36012
2004
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1
3
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2
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1
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3
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1
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1
1
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2
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1
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1
3
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2
1
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1
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1
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1
1
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2
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645962
Ricagno
Crystallization and preliminar ...
Oxalobacter formigenes
Acta Crystallogr. Sect. D
59
1276-1277
2003
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1
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1
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3
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1
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1
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1
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1
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1
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1
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645963
Ricagno
Formyl-CoA transferase enclose ...
Oxalobacter formigenes
EMBO J.
22
3210-3219
2003
-
-
1
1
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1
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4
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1
1
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3
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4
1
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1
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1
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1
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1
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3
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4
1
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645961
Sidhu
DNA sequencing and expression ...
Oxalobacter formigenes
J. Bacteriol.
179
3378-3381
1997
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1
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1
1
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4
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1
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1
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1
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1
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1
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3
1
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645960
Baetz
-
Localization of oxalyl-coenzym ...
Oxalobacter formigenes, Oxalobacter formigenes OxB / ATCC 35274
Syst. Appl. Microbiol.
15
167-171
1992
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1
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2
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645959
Baetz
Purification and characterizat ...
Oxalobacter formigenes
J. Bacteriol.
172
3537-3540
1990
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2
3
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2
1
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2
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1
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1
1
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