BRENDA - Enzyme Database
show all sequences of 2.8.3.16

Transcriptional and functional analysis of oxalyl-coenzyme A (CoA) decarboxylase and formyl-CoA transferase genes from Lactobacillus acidophilus

Azcarate-Peril, M.A.; Bruno-Barcena, J.M.; Hassan, H.M.; Klaenhammer, T.R.; Appl. Environ. Microbiol. 72, 1891-1899 (2006)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
oxalate induces the expression of genes frc and oxc
Lactobacillus acidophilus
Cloned(Commentary)
Cloned (Commentary)
Organism
gene frc, DNA and amino acid sequence determination and analysis, phylogenetic analysis
Bifidobacterium animalis subsp. lactis
gene frc, DNA and amino acid sequence determination and analysis, phylogenetic analysis
Lactobacillus gasseri
gene frc, organized in the oxc operon, DNA and amino acid sequence determination and analysis, transcriptional and quantitative expression analysis, mildly acidic conditions are a prerequisite for frc and oxc transcription, phylogenetic analysis
Lactobacillus acidophilus
Engineering
Protein Variants
Commentary
Organism
additional information
construction of a frc deletion mutant, frc expression specifically improves survival in the presence of oxalic acid at pH 3.5, the frc mutant is unable to degrade oxalate, phenotype, overview
Lactobacillus acidophilus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
formyl-CoA + oxalate
Lactobacillus acidophilus
the enzyme is required for oxalate degradation together with oxalyl-CoA decarboxylase, EC 4.1.1.8, overview
formate + oxalyl-CoA
-
-
?
formyl-CoA + oxalate
Lactobacillus acidophilus NCFM
the enzyme is required for oxalate degradation together with oxalyl-CoA decarboxylase, EC 4.1.1.8, overview
formate + oxalyl-CoA
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Bifidobacterium animalis subsp. lactis
-
gene frc
-
Lactobacillus acidophilus
-
-
-
Lactobacillus acidophilus NCFM
-
-
-
Lactobacillus gasseri
-
gene frc
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
formyl-CoA + oxalate
-
671452
Lactobacillus acidophilus
formate + oxalyl-CoA
-
-
-
?
formyl-CoA + oxalate
the enzyme is required for oxalate degradation together with oxalyl-CoA decarboxylase, EC 4.1.1.8, overview
671452
Lactobacillus acidophilus
formate + oxalyl-CoA
-
-
-
?
formyl-CoA + oxalate
-
671452
Lactobacillus acidophilus NCFM
formate + oxalyl-CoA
-
-
-
?
formyl-CoA + oxalate
the enzyme is required for oxalate degradation together with oxalyl-CoA decarboxylase, EC 4.1.1.8, overview
671452
Lactobacillus acidophilus NCFM
formate + oxalyl-CoA
-
-
-
?
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
oxalate induces the expression of genes frc and oxc
Lactobacillus acidophilus
Cloned(Commentary) (protein specific)
Commentary
Organism
gene frc, DNA and amino acid sequence determination and analysis, phylogenetic analysis
Bifidobacterium animalis subsp. lactis
gene frc, DNA and amino acid sequence determination and analysis, phylogenetic analysis
Lactobacillus gasseri
gene frc, organized in the oxc operon, DNA and amino acid sequence determination and analysis, transcriptional and quantitative expression analysis, mildly acidic conditions are a prerequisite for frc and oxc transcription, phylogenetic analysis
Lactobacillus acidophilus
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
construction of a frc deletion mutant, frc expression specifically improves survival in the presence of oxalic acid at pH 3.5, the frc mutant is unable to degrade oxalate, phenotype, overview
Lactobacillus acidophilus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
formyl-CoA + oxalate
Lactobacillus acidophilus
the enzyme is required for oxalate degradation together with oxalyl-CoA decarboxylase, EC 4.1.1.8, overview
formate + oxalyl-CoA
-
-
?
formyl-CoA + oxalate
Lactobacillus acidophilus NCFM
the enzyme is required for oxalate degradation together with oxalyl-CoA decarboxylase, EC 4.1.1.8, overview
formate + oxalyl-CoA
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
formyl-CoA + oxalate
-
671452
Lactobacillus acidophilus
formate + oxalyl-CoA
-
-
-
?
formyl-CoA + oxalate
the enzyme is required for oxalate degradation together with oxalyl-CoA decarboxylase, EC 4.1.1.8, overview
671452
Lactobacillus acidophilus
formate + oxalyl-CoA
-
-
-
?
formyl-CoA + oxalate
-
671452
Lactobacillus acidophilus NCFM
formate + oxalyl-CoA
-
-
-
?
formyl-CoA + oxalate
the enzyme is required for oxalate degradation together with oxalyl-CoA decarboxylase, EC 4.1.1.8, overview
671452
Lactobacillus acidophilus NCFM
formate + oxalyl-CoA
-
-
-
?
Other publictions for EC 2.8.3.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739759
Herve
Diversity and ecology of oxalo ...
Actinobacteria, Firmicutes, Proteobacteria
World J. Microbiol. Biotechnol.
32
28
2016
-
-
-
-
-
-
-
-
-
-
-
3
-
8
-
-
-
-
-
-
-
-
3
-
6
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726452
Mullins
Formyl-coenzyme A (CoA):oxalat ...
Acetobacter aceti 1023, Acetobacter aceti
Protein Sci.
21
686-696
2012
-
-
1
1
-
-
-
2
1
-
4
2
-
4
-
-
1
-
-
-
-
-
2
1
3
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
1
-
4
2
-
-
-
1
-
-
-
-
2
1
-
-
-
2
-
-
-
-
1
-
-
1
2
2
692851
Toyota
Differential substrate specifi ...
Escherichia coli, Escherichia coli MG1655, Oxalobacter formigenes
J. Bacteriol.
190
2556-2564
2008
-
-
2
1
2
-
4
10
-
-
-
3
-
32
-
-
2
-
-
-
-
-
6
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
1
2
-
-
4
2
10
-
-
-
3
-
-
-
2
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693216
Berthold
Reinvestigation of the catalyt ...
Oxalobacter formigenes
J. Biol. Chem.
283
6519-6529
2008
-
-
1
1
3
-
2
8
-
-
-
1
-
2
-
-
1
-
-
-
-
1
2
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
1
3
-
-
2
2
8
-
-
-
1
-
-
-
1
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693608
Khammar
Use of the frc gene as a molec ...
Ancylobacter oerskovii, Ancylobacter polymorphus, Arquibacter sp., Azorhizobium sp., Azospirillum brasilense, Azospirillum lipoferum, Bradyrhizobium japonicum, Bradyrhizobium sp., Cupriavidus necator, Cupriavidus necator JMP 134-1, Cupriavidus oxalaticus, Escherichia coli, Herminiimonas arsenicoxydans, Herminiimonas saxobsidens, Janthinobacterium sp. Marseille, Methylobacterium organophilum, Methylorubrum extorquens, Methylorubrum thiocyanatum, Oxalicibacterium flavum, Oxalobacter formigenes, Pandoraea sp., Paraburkholderia xenovorans, Rhodopseudomonas palustris, Shigella flexneri, Starkeya novella, Streptomyces avermitilis, Streptomyces coelicolor, Streptomyces violaceoruber, Variovorax paradoxus, Xanthobacter autotrophicus, Xanthobacter flavus, Xanthomonas sp.
J. Microbiol. Methods
76
120-127
2008
-
93
31
-
-
-
-
-
-
-
-
36
-
39
-
-
-
-
-
62
-
-
72
-
35
-
-
-
-
-
-
-
-
-
-
-
-
105
35
-
-
-
-
-
-
-
-
-
-
-
36
-
-
-
-
-
70
-
-
72
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674225
Turroni
Oxalate consumption by lactoba ...
Lactobacillus acidophilus
J. Appl. Microbiol.
103
1600-1609
2007
-
-
1
-
-
-
-
-
-
-
1
1
-
5
-
-
-
-
-
-
-
-
2
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
692373
Lewanika
Lactobacillus gasseri Gasser A ...
Lactobacillus gasseri, Lactobacillus gasseri Gasser AM63T
FEMS Microbiol. Ecol.
61
110-120
2007
-
1
-
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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2
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-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
692677
Ye
Stable expression of the oxc a ...
Oxalobacter formigenes
Int. J. Mol. Med.
20
521-526
2007
-
1
1
-
-
-
-
-
1
-
-
1
-
4
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671452
Azcarate-Peril
Transcriptional and functional ...
Bifidobacterium animalis subsp. lactis, Lactobacillus acidophilus, Lactobacillus acidophilus NCFM, Lactobacillus gasseri
Appl. Environ. Microbiol.
72
1891-1899
2006
1
-
3
-
1
-
-
-
-
-
-
2
-
14
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662253
Jonsson
Kinetic and mechanistic charac ...
Oxalobacter formigenes
J. Biol. Chem.
279
36003-36012
2004
-
-
-
1
3
-
2
-
-
-
1
-
-
3
-
-
1
-
-
-
-
-
1
1
1
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
1
3
-
-
2
1
-
-
-
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
645962
Ricagno
Crystallization and preliminar ...
Oxalobacter formigenes
Acta Crystallogr. Sect. D
59
1276-1277
2003
-
-
-
1
-
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645963
Ricagno
Formyl-CoA transferase enclose ...
Oxalobacter formigenes
EMBO J.
22
3210-3219
2003
-
-
1
1
-
-
-
-
-
-
-
1
-
4
-
-
1
1
-
3
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
3
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645961
Sidhu
DNA sequencing and expression ...
Oxalobacter formigenes
J. Bacteriol.
179
3378-3381
1997
-
-
1
-
-
-
-
-
-
-
1
1
-
4
-
-
1
-
-
-
-
-
3
1
-
-
-
-
-
-
-
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-
-
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1
-
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-
-
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1
1
-
-
-
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645960
Baetz
-
Localization of oxalyl-coenzym ...
Oxalobacter formigenes, Oxalobacter formigenes OxB / ATCC 35274
Syst. Appl. Microbiol.
15
167-171
1992
-
-
-
-
-
-
-
-
1
-
-
2
-
2
-
-
1
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1
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2
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1
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1
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1
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645959
Baetz
Purification and characterizat ...
Oxalobacter formigenes
J. Bacteriol.
172
3537-3540
1990
-
-
-
-
-
-
2
3
-
-
2
1
-
2
-
-
1
-
-
-
1
-
3
1
-
1
-
-
-
1
-
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1
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-
-
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-
-
-
-
-
2
-
3
-
-
2
1
-
-
-
1
-
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1
-
3
1
1
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-
-
1
-
-
1
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-
-