BRENDA - Enzyme Database
show all sequences of 2.8.3.16

Purification and characterization of formyl-coenzyme A transferase from Oxalobacter formigenes

Baetz, A.L.; Allison, M.J.; J. Bacteriol. 172, 3537-3540 (1990)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
N-ethylmaleimide
20% inhibition at 1 mM
Oxalobacter formigenes
p-chloromercuribenzoate
91% inhibition at 1 mM, causes precipitation
Oxalobacter formigenes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3
-
succinate
pH 6.7, 25°C
Oxalobacter formigenes
3
-
formyl-CoA
pH 6.7, 25°C
Oxalobacter formigenes
5.1
-
oxalate
pH 6.7, 25°C
Oxalobacter formigenes
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
1 * 44000, SDS-PAGE
Oxalobacter formigenes
44700
-
gel filtration
Oxalobacter formigenes
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
formyl-CoA + oxalate
Oxalobacter formigenes
-
formate + oxalyl-CoA
-
Oxalobacter formigenes
?
Organism
Organism
UniProt
Commentary
Textmining
Oxalobacter formigenes
O06644
-
-
Purification (Commentary)
Purification (Commentary)
Organism
31.2fold, to homogeneity
Oxalobacter formigenes
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.15
-
purified enyzme
Oxalobacter formigenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
formyl-CoA + oxalate
-
645959
Oxalobacter formigenes
formate + oxalyl-CoA
-
645959
Oxalobacter formigenes
?
additional information
no activity with acetate or malonate
645959
Oxalobacter formigenes
?
-
-
-
-
succinyl-CoA + oxalate
11% of the reaction rate observed with formyl-CoA
645959
Oxalobacter formigenes
succinate + oxalyl-CoA
-
645959
Oxalobacter formigenes
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 44000, SDS-PAGE
Oxalobacter formigenes
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Oxalobacter formigenes
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7.5
-
Oxalobacter formigenes
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Oxalobacter formigenes
-
4.8
4.7
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
N-ethylmaleimide
20% inhibition at 1 mM
Oxalobacter formigenes
p-chloromercuribenzoate
91% inhibition at 1 mM, causes precipitation
Oxalobacter formigenes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3
-
succinate
pH 6.7, 25°C
Oxalobacter formigenes
3
-
formyl-CoA
pH 6.7, 25°C
Oxalobacter formigenes
5.1
-
oxalate
pH 6.7, 25°C
Oxalobacter formigenes
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
1 * 44000, SDS-PAGE
Oxalobacter formigenes
44700
-
gel filtration
Oxalobacter formigenes
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
formyl-CoA + oxalate
Oxalobacter formigenes
-
formate + oxalyl-CoA
-
Oxalobacter formigenes
?
Purification (Commentary) (protein specific)
Commentary
Organism
31.2fold, to homogeneity
Oxalobacter formigenes
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.15
-
purified enyzme
Oxalobacter formigenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
formyl-CoA + oxalate
-
645959
Oxalobacter formigenes
formate + oxalyl-CoA
-
645959
Oxalobacter formigenes
?
additional information
no activity with acetate or malonate
645959
Oxalobacter formigenes
?
-
-
-
-
succinyl-CoA + oxalate
11% of the reaction rate observed with formyl-CoA
645959
Oxalobacter formigenes
succinate + oxalyl-CoA
-
645959
Oxalobacter formigenes
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 44000, SDS-PAGE
Oxalobacter formigenes
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Oxalobacter formigenes
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7.5
-
Oxalobacter formigenes
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Oxalobacter formigenes
-
4.8
4.7
Other publictions for EC 2.8.3.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739759
Herve
Diversity and ecology of oxalo ...
Actinobacteria, Firmicutes, Proteobacteria
World J. Microbiol. Biotechnol.
32
28
2016
-
-
-
-
-
-
-
-
-
-
-
3
-
8
-
-
-
-
-
-
-
-
3
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726452
Mullins
Formyl-coenzyme A (CoA):oxalat ...
Acetobacter aceti 1023, Acetobacter aceti
Protein Sci.
21
686-696
2012
-
-
1
1
-
-
-
2
1
-
4
2
-
4
-
-
1
-
-
-
-
-
2
1
3
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
1
-
4
2
-
-
-
1
-
-
-
-
2
1
-
-
-
2
-
-
-
-
1
-
-
1
2
2
692851
Toyota
Differential substrate specifi ...
Escherichia coli, Escherichia coli MG1655, Oxalobacter formigenes
J. Bacteriol.
190
2556-2564
2008
-
-
2
1
2
-
4
10
-
-
-
3
-
32
-
-
2
-
-
-
-
-
6
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
1
2
-
-
4
2
10
-
-
-
3
-
-
-
2
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693216
Berthold
Reinvestigation of the catalyt ...
Oxalobacter formigenes
J. Biol. Chem.
283
6519-6529
2008
-
-
1
1
3
-
2
8
-
-
-
1
-
2
-
-
1
-
-
-
-
1
2
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
1
3
-
-
2
2
8
-
-
-
1
-
-
-
1
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693608
Khammar
Use of the frc gene as a molec ...
Ancylobacter oerskovii, Ancylobacter polymorphus, Arquibacter sp., Azorhizobium sp., Azospirillum brasilense, Azospirillum lipoferum, Bradyrhizobium japonicum, Bradyrhizobium sp., Cupriavidus necator, Cupriavidus necator JMP 134-1, Cupriavidus oxalaticus, Escherichia coli, Herminiimonas arsenicoxydans, Herminiimonas saxobsidens, Janthinobacterium sp. Marseille, Methylobacterium organophilum, Methylorubrum extorquens, Methylorubrum thiocyanatum, Oxalicibacterium flavum, Oxalobacter formigenes, Pandoraea sp., Paraburkholderia xenovorans, Rhodopseudomonas palustris, Shigella flexneri, Starkeya novella, Streptomyces avermitilis, Streptomyces coelicolor, Streptomyces violaceoruber, Variovorax paradoxus, Xanthobacter autotrophicus, Xanthobacter flavus, Xanthomonas sp.
J. Microbiol. Methods
76
120-127
2008
-
93
31
-
-
-
-
-
-
-
-
36
-
39
-
-
-
-
-
62
-
-
72
-
35
-
-
-
-
-
-
-
-
-
-
-
-
105
35
-
-
-
-
-
-
-
-
-
-
-
36
-
-
-
-
-
70
-
-
72
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674225
Turroni
Oxalate consumption by lactoba ...
Lactobacillus acidophilus
J. Appl. Microbiol.
103
1600-1609
2007
-
-
1
-
-
-
-
-
-
-
1
1
-
5
-
-
-
-
-
-
-
-
2
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
692373
Lewanika
Lactobacillus gasseri Gasser A ...
Lactobacillus gasseri, Lactobacillus gasseri Gasser AM63T
FEMS Microbiol. Ecol.
61
110-120
2007
-
1
-
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
692677
Ye
Stable expression of the oxc a ...
Oxalobacter formigenes
Int. J. Mol. Med.
20
521-526
2007
-
1
1
-
-
-
-
-
1
-
-
1
-
4
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671452
Azcarate-Peril
Transcriptional and functional ...
Bifidobacterium animalis subsp. lactis, Lactobacillus acidophilus, Lactobacillus acidophilus NCFM, Lactobacillus gasseri
Appl. Environ. Microbiol.
72
1891-1899
2006
1
-
3
-
1
-
-
-
-
-
-
2
-
14
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662253
Jonsson
Kinetic and mechanistic charac ...
Oxalobacter formigenes
J. Biol. Chem.
279
36003-36012
2004
-
-
-
1
3
-
2
-
-
-
1
-
-
3
-
-
1
-
-
-
-
-
1
1
1
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
1
3
-
-
2
1
-
-
-
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
645962
Ricagno
Crystallization and preliminar ...
Oxalobacter formigenes
Acta Crystallogr. Sect. D
59
1276-1277
2003
-
-
-
1
-
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645963
Ricagno
Formyl-CoA transferase enclose ...
Oxalobacter formigenes
EMBO J.
22
3210-3219
2003
-
-
1
1
-
-
-
-
-
-
-
1
-
4
-
-
1
1
-
3
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
3
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645961
Sidhu
DNA sequencing and expression ...
Oxalobacter formigenes
J. Bacteriol.
179
3378-3381
1997
-
-
1
-
-
-
-
-
-
-
1
1
-
4
-
-
1
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645960
Baetz
-
Localization of oxalyl-coenzym ...
Oxalobacter formigenes, Oxalobacter formigenes OxB / ATCC 35274
Syst. Appl. Microbiol.
15
167-171
1992
-
-
-
-
-
-
-
-
1
-
-
2
-
2
-
-
1
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1
-
2
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1
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2
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1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645959
Baetz
Purification and characterizat ...
Oxalobacter formigenes
J. Bacteriol.
172
3537-3540
1990
-
-
-
-
-
-
2
3
-
-
2
1
-
2
-
-
1
-
-
-
1
-
3
1
-
1
-
-
-
1
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1
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2
-
3
-
-
2
1
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-
-
1
-
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1
-
3
1
1
-
-
-
1
-
-
1
-
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-
-
-