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Literature summary for 2.8.3.15 extracted from

  • Schuehle, K.; Nies, J.; Heider, J.
    An indoleacetate-CoA ligase and a phenylsuccinyl-CoA transferase involved in anaerobic metabolism of auxin (2016), Environ. Microbiol., 18, 3120-3132.
    View publication on PubMed
Search for more literature for 2.8.3.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Aromatoleum aromaticum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
-
 Aromatoleum aromaticum

Organism

Organism UniProt Comment Textmining
Aromatoleum aromaticum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
strep-tactin affinity column chromatography Aromatoleum aromaticum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.024
-
 with (R/S)-phenylsuccinyl-CoA and (R,S)-benzylsuccinate as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
0.085
-
 with succinyl-CoA and (S)-benzylsuccinate as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
0.103
-
 with succinyl-CoA and (R,S)-benzylsuccinate as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
0.108
-
 with (R/S)-phenylsuccinyl-CoA and (R,S)-phenylsuccinate as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
0.122
-
 with succinyl-CoA and (R)-benzylsuccinate as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
0.136
-
 with succinyl-CoA and 2-oxo-indole-3-acetate as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
0.188
-
 with succinyl-CoA and indole-3-acetate as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
0.235
-
 with succinyl-CoA and(R,S)-phenylsuccinate as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R/S)-phenylsuccinyl-CoA + (R,S)-benzylsuccinate 10% activity compared to succinyl-CoA plus (R,S)-phenylsuccinate Aromatoleum aromaticum (R/S)-phenylsuccinate + (R,S)-benzylsuccinyl-CoA
-
 ?
(R/S)-phenylsuccinyl-CoA + (R,S)-phenylsuccinate 46% activity compared to succinyl-CoA plus (R,S)-phenylsuccinate Aromatoleum aromaticum (R/S)-phenylsuccinate + (R,S)-phenylsuccinyl-CoA
-
 ?
additional information the enzyme shows no activity with benzylmalonate, acetyl-CoA, and cinnamate Aromatoleum aromaticum ?
-
 ?
succinyl-CoA + (R)-benzylsuccinate 52% activity compared to succinyl-CoA plus (R,S)-phenylsuccinate Aromatoleum aromaticum succinate + (R)-benzylsuccinyl-CoA
-
 ?
succinyl-CoA + (R,S)-benzylsuccinate 44% activity compared to succinyl-CoA plus (R,S)-phenylsuccinate Aromatoleum aromaticum succinate + (R,S)-benzylsuccinyl-CoA
-
 ?
succinyl-CoA + (R,S)-phenylsuccinate 100% activity Aromatoleum aromaticum succinate + 2-phenylsuccinyl-CoA + 3-phenylsuccinyl-CoA
-
 ?
succinyl-CoA + (S)-benzylsuccinate 36% activity compared to succinyl-CoA plus (R,S)-phenylsuccinate Aromatoleum aromaticum succinate + (S)-benzylsuccinyl-CoA
-
 ?
succinyl-CoA + 2-oxoindole-3-acetate 58% activity compared to succinyl-CoA plus (R,S)-phenylsuccinate Aromatoleum aromaticum succinate + 2-oxoindole-3-acetyl-CoA
-
 ?
succinyl-CoA + indole-3-acetate 80% activity compared to succinyl-CoA plus (R,S)-phenylsuccinate Aromatoleum aromaticum succinate + indole-3-acetyl-CoA
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, Strep-tagged enzyme, SDS-PAGE Aromatoleum aromaticum

Synonyms

Synonyms Comment Organism
IaaL
-
 Aromatoleum aromaticum
phenylsuccinyl-CoA transferase
-
 Aromatoleum aromaticum
succinyl-CoA:phenylsuccinate CoA-transferase
-
 Aromatoleum aromaticum