Cloned (Comment) | Organism |
---|---|
GCT expression in Escherichia coli | Acidaminococcus fermentans |
Crystallization (Comment) | Organism |
---|---|
recombinant GCT, expressed in Escherichia coli, sitting-drop vapour diffusion method | Acidaminococcus fermentans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
alpha4beta4, 4 * 35000 + 4 * 29000, structure and association of the subunits A and B | Acidaminococcus fermentans |
35000 | - |
alpha4beta4, 4 * 35000 + 4 * 29000, structure and association of the subunits A and B | Acidaminococcus fermentans |
260000 | - |
heterooctamer GCT | Acidaminococcus fermentans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + (R)-2-hydroxyglutarate | Acidaminococcus fermentans | in the course of glutamate fermentation | acetate + (R)-2-hydroxyglutaryl-CoA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidaminococcus fermentans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GCT, expressed in Escherichia coli | Acidaminococcus fermentans |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + (E)-glutaconate = acetate + glutaconyl-1-CoA | ping-pong mechanism | Acidaminococcus fermentans | |
acetyl-CoA + (E)-glutaconate = acetate + glutaconyl-1-CoA | reaction mechanism | Acidaminococcus fermentans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + (E)-glutaconate | - |
Acidaminococcus fermentans | acetate + glutaconyl-1-CoA | - |
r | |
acetyl-CoA + (R)-2-hydroxyglutarate | - |
Acidaminococcus fermentans | acetate + (R)-2-hydroxyglutaryl-1-CoA | - |
r | |
acetyl-CoA + (R)-2-hydroxyglutarate | in the course of glutamate fermentation | Acidaminococcus fermentans | acetate + (R)-2-hydroxyglutaryl-CoA | - |
r | |
additional information | GCT structure, the active site is located at the interface of subunits A and B and is formed by loops of both subunits | Acidaminococcus fermentans | ? | - |
? | |
additional information | catalytic residue is E-54 of subunit B, catalytic mechanism | Acidaminococcus fermentans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | alpha4beta4, 4 * 35000 + 4 * 29000, structure and association of the subunits A and B | Acidaminococcus fermentans |