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Literature summary for 2.8.2.B6 extracted from
- Changes in heparan sulfate sulfotransferases and cell-surface heparan sulfate during SKM-1 cells granulocytic differentiation and A549 cells epithelial-mesenchymal transition (2020), Glycoconj. J., 37, 151-164 .
Application
| Application | Comment | Organism |
|---|---|---|
| diagnostics | specific HS sulfotransferases may serve as molecular markers and targets for cancer treatment | Homo sapiens |
| medicine | specific HS sulfotransferases may serve as molecular markers and targets for cancer treatment | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene HS2ST1, quantitative RT-PCR enzyme expression analysis | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi apparatus | - |
Homo sapiens | 5794 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + [heparan sulfate]-iduronate | Homo sapiens | - |
adenosine 3',5'-bisphosphate + [heparan sulfate]-iduronate 2-sulfate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q7LGA3 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| A-549 cell | - |
Homo sapiens | - |
| epithelial cell | - |
Homo sapiens | - |
| granulocyte | - |
Homo sapiens | - |
| lung cancer cell | - |
Homo sapiens | - |
| additional information | changes in heparan sulfate sulfotransferases and cell-surface heparan sulfate occur during SKM-1 cells granulocytic differentiation and A-549 cells epithelial-mesenchymal transition. Results of RT-PCR show that ndst1, ndst2, hs2st1, hs6st1, hs6st3, hs3st3a, hs3st3b and hs3st5 mRNA are expressed during A549 cells EMT. And the other members in different sites-modifying gene families including ndst3, ndst4, hs6st2, hs3st1, hs3st2 and hs3st4 mRNA are not detected with at least two different pairs of primers and in three different PCR conditions | Homo sapiens | - |
| non-small cell lung cancer cell | - |
Homo sapiens | - |
| SKM-1 cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + [heparan sulfate]-iduronate | - |
Homo sapiens | adenosine 3',5'-bisphosphate + [heparan sulfate]-iduronate 2-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + [heparan sulfate]-iduronate | sulfotransferase HS2ST1 performs 2-O sulfation of iduronic acid in heparan sulfate | Homo sapiens | adenosine 3',5'-bisphosphate + [heparan sulfate]-iduronate 2-sulfate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| heparan sulfate sulfotransferase | - |
Homo sapiens |
| HS 2-O sulfotransferase 1 | - |
Homo sapiens |
| HS2ST1 | - |
Homo sapiens |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Homo sapiens | HS 2-O sulfotransferase 1 (HS2ST1) is downregulated at both mRNA and protein levels during granulocytic differentiation of SKM-1 leukemia cells and also HS (glucosamine) 3-O sulfotransferase 3A (HS3ST3A) in epithelial-mesenchymal transition (EMT) of A-549 lung cancer cells, meanwhile, cell-surface HS recognized by anti-HS antibody is also changed in both cancer cell lines. HS3ST3A (EC 2.8.2.30) is negatively correlated with the in vitro cell metastasis capability of A549 cells confirmed by RNA interference technology, wound-healing assay and in vitro Matrigel invasion assay | down |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | there are several isoforms in every gene family except that HS2ST gene family has only one isoform | Homo sapiens |
| metabolism | heparan sulfate (HS) is a type of glycosaminoglycan consisting of variably sulfated glucuronic acid or iduronic acid-N-acetylglucosamine repeating disaccharide units, and it can bind plenty of growth factors such as FGF2 and its receptor FGFR1, thereby regulating cancer cell signaling. Divergent fine structures of HS are generated by sulfation catalyzed by HS N-deacetylase/N-, 2-O-, 6-O-, 3-O-sulfotransferases (correspondingly abbreviated as NDST, HS2ST, HS6ST, HS3ST), epimerization catalyzed by HS C5-epimerase at the Golgi lumen and further desulfation catalyzed by HS 6-O-endosulfatase at the cell surface. There are several isoforms in every gene family except that HS2ST gene family has only one isoform | Homo sapiens |
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