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Literature summary for 2.8.2.B6 extracted from

  • Teixeira, F.; Vijaya Kumar, A.; Kumar Katakam, S.; Cocola, C.; Pelucchi, P.; Graf, M.; Kiesel, L.; Reinbold, R.; Pavao, M.; Greve, B.; Goette, M.
    The heparan sulfate sulfotransferases HS2ST1 and HS3ST2 are novel regulators of breast Cancer stem-cell Properties (2020), Front. Cell Dev. Biol., 8, 559554 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
gene HS2ST1, quantitative real-time PCR enzyme expression analysis, recombinant overexpression in MDA-MB-231 and MCF-7 cells, transcriptome analysis, overview. The expression levels of several genes are altered by overexpression of HS2ST1 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl sulfate + [heparan sulfate]-iduronate Homo sapiens sulfotransferase HS2ST1 performs 2-O sulfation of iduronic acid in heparan sulfate adenosine 3',5'-bisphosphate + [heparan sulfate]-iduronate 2-sulfate
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Organism

Organism UniProt Comment Textmining
Homo sapiens Q7LGA3
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Source Tissue

Source Tissue Comment Organism Textmining
breast cancer cell
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 Homo sapiens
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cancer stem cell CSC Homo sapiens
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MCF-7 cell
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 Homo sapiens
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MDA-MB-231 cell
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 Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + [heparan sulfate]-iduronate sulfotransferase HS2ST1 performs 2-O sulfation of iduronic acid in heparan sulfate Homo sapiens adenosine 3',5'-bisphosphate + [heparan sulfate]-iduronate 2-sulfate
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Synonyms

Synonyms Comment Organism
HS2ST1
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 Homo sapiens

General Information

General Information Comment Organism
malfunction HS2ST1 expression is dysregulated in several tumor entities, suggesting a role in tumor progression. Influence of HS2ST1 overexpression on the CSC phenotype in breast cancer cell lines representative of the triple-negative (MDA-MB-231) and hormone-receptor positive subtype (MCF-7). The CD44+/CD24-/low phenotype is significantly reduced in MDA-MB-231 cells after overexpression of the enzyme, remaining unaltered in MCF-7 cells. Colony and spheroid formation are increased after HS2ST1 overexpression in MCF-7 cells. MDA-MB-231 cells overexpressing HS2ST1 form more colonies and can not generate spheres. The phenotypic changes are associated with complex changes in the expression of the stemness-associated notch and Wnt-signaling pathways constituents, syndecans, heparanase and Sulf1. Heparan sulfotransferase subtype-specific impact of HS modifications on the CSC phenotype of triple-negative and hormone receptor positive breast cancer model cell lines. In MDA-MB-231 cells, ALDH1 activity increased from 2.88% in the control transfected cells to 9.99% after overexpression of HS2ST1 overexpressing cells. HS2ST1 overexpression in MCF-7 cells decreases the activity of ALDH from 4.42% in the control transfected cells to 0.91% after its overexpression Homo sapiens
metabolism high sulfation degree of heparan mediates functional interactions with positively charged amino acids in proteins. 2-O sulfation of iduronic acid and 3-O sulfation of glucosamine in heparan sulfate (HS) are mediated by the sulfotransferases HS2ST and HS3ST, respectively, which are dysregulated in several cancers. Both sulfotransferases regulate breast cancer cell viability and invasion. The syndecan family of HSPGs and the Notch pathway are part of the regulatory circuit of HS sulfotransferases. The expression levels of several genes are altered by overexpression of HS2ST1, breast cancer cell transcriptome analysis, overview Homo sapiens
physiological function the sulfotransferase HS2ST1 regulates breast cancer cell viability and invasion. It catalyzes the 2-O sulfation of iduronic acid in heparan sulfate. The 2-O sulfated iduronic acid (IdoA2S) is a widely common heparan sulfate (HS) motif and mediates the binding and signaling of several growth factors Homo sapiens