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Literature summary for 2.8.2.B1 extracted from

  • Li, J.; Schiff, J.A.
    Purification and properties of adenosine 5'-phosphosulphate sulphotransferase from Euglena (1991), Biochem. J., 274, 355-360.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.B1

Activating Compound

Activating Compound Comment Organism Structure
EDTA
-
 Euglena sp.
EDTA 2.0 mM, 1.7fold stimulation Euglena gracilis

General Stability

General Stability Organism
adenosine 5'-phosphoramidate protects the enzyme from progressive inactivation by thiol substrates Euglena gracilis

Inhibitors

Inhibitors Comment Organism Structure
2,3-Dimercaptopropan-1-ol
-
 Euglena sp.
2-mercaptoethanol
-
 Euglena sp.
adenosine 5'-phosphoramidate 0.5 mM, inhibits after 2 min incubation, activates after 20 min incubation Euglena gracilis
adenosine 5'-sulfate 2.0 mM, inhibits after 2 min incubation, activates after 20 min incubation Euglena gracilis
AMP 2.0 mM, inhibits after 2 min incubation, activates after 20 min incubation Euglena gracilis
CoCl2 2 mM, 50% inhibition Euglena gracilis
Cu2+
-
 Euglena sp.
CuCl2 0.1 mM, 50% inhibition Euglena gracilis
cysteamine hydrochloride
-
 Euglena sp.
dithioerythritol
-
 Euglena sp.
dithiothreitol
-
 Euglena sp.
Fe2+
-
 Euglena sp.
FeCl2 2 mM, 50% inhibition Euglena gracilis
Hg2+
-
 Euglena sp.
HgCl2 0.1 mM, 50% inhibition Euglena gracilis
KCN
-
 Euglena sp.
L-cysteine
-
 Euglena sp.
L-glutathione
-
 Euglena sp.
L-homocysteine
-
 Euglena sp.
SO32-
-
 Euglena sp.
Sodium thioglycollate
-
 Euglena sp.
sodium thiosulfate
-
 Euglena sp.
Thioacetamide
-
 Euglena sp.
Zn2+
-
 Euglena sp.
ZnCl2 2 mM, 50% inhibition Euglena gracilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0001
-
 adenosine 5'-phosphosulfate 25°C, pH 9.0, reaction with dithiothreitol Euglena gracilis

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial inner membrane periplasmic side Euglena sp. 5743
-

Metals/Ions

Metals/Ions Comment Organism Structure
adenosine 5'-phosphoramidate 0.5 mM, inhibits after 2 min incubation, activates after 20 min incubation. Inhibits by decreasing the initial velocity of the reaction as adenosine 5'-phosphoramidate concentration is increased Euglena gracilis
Ba2+
-
 Euglena sp.
BaCl2 30 mM, 3fold stimulation Euglena gracilis
Ca2+
-
 Euglena sp.
CaCl2 30 mM, 3fold stimulation Euglena gracilis
Mg2+
-
 Euglena sp.
MgCl2 30 mM, 3fold stimulation Euglena gracilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 gel filtration Euglena gracilis
120000
-
 gel filtration Euglena sp.

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
 Klebs var. bacillaris mutant W10BSmL
-
Euglena sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Euglena gracilis
-
 Euglena sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.14
-
-
 Euglena sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine 5'-phosphosulfate + dithioerythritol
-
 Euglena gracilis sulfite + ?
-
 ?
adenosine 5'-phosphosulfate + dithiothreitol
-
 Euglena gracilis sulfite + ?
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Euglena sp.
tetramer held together by covalent (probably disulphide) bonds Euglena gracilis

Synonyms

Synonyms Comment Organism
adenosine 5'-phosphosulfate sulfotransferase
-
 Euglena gracilis
APSST
-
 Euglena gracilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Euglena sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Euglena sp.

pI Value

Organism Comment pI Value Maximum pI Value
Euglena gracilis isoelectric focusing, the enzyme may exist in multiple forms having different pI values 5.5 5