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Literature summary for 2.8.2.9 extracted from

  • Marshall, A.D.; Darbyshire, J.F.; McPhie, P.; Jakoby, W.B.
    A review of the effects of manipulation of the cysteine residues of rat aryl sulfotransferase IV (1998), Chem. Biol. Interact., 109, 107-116.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) low concentrations lead to increase of activity at a physiological pH, higher concentrations lead to complete inactivation Rattus norvegicus
Diamide low concentrations lead to increase of activity at a physiological pH, higher concentrations lead to complete inactivation Rattus norvegicus
glutathione disulfide leads to the formation of internal disulfide bonds between Cys66 and Cys232, activates the enzyme and allows it to function at a pH optimum in the physiological range Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
-
Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
C232S mutant is catalytically active Rattus norvegicus
C66S mutant is catalytically active, can not be activated by glutathione disulfide Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Phenylarsine oxide effective inhibitor Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + 2-naphthol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
-
?
3'-phosphoadenylylsulfate + 4-nitrophenol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pH optima for sulfation are dependent on the oxidation state of the sulfotransferase Rattus norvegicus
5.4
-
-
Rattus norvegicus
6
-
two optima: pH 6 and pH 9.7, reduced enzyme activity with 2-naphthylamine as substrate Rattus norvegicus
8
-
oxidized enzyme Rattus norvegicus
9.7
-
two optima: pH 6 and pH 9.7, reduced activity with 2-naphthylamine as substrate Rattus norvegicus