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Literature summary for 2.8.2.8 extracted from
- Mast cell differentiation and activation is closely linked to expression of genes coding for the serglycin proteoglycan core protein and a distinct set of chondroitin sulfate and heparin sulfotransferases (2009), J. Immunol., 183, 7073-7083.
Application
| Application | Comment | Organism |
|---|---|---|
| diagnostics | NDST-2 expression is a late differentiation marker for mast cells | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine | Mus musculus | the sulfation of heparin is initiated by the NDST enzymes, which catalyze the N-deacetylation and subsequent N-sulfation of selected GlcNAc residues of the [GlcUA-GlcNAc] polymer | adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine | - |
? |  |
| 3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine | Mus musculus C57BL/6 | the sulfation of heparin is initiated by the NDST enzymes, which catalyze the N-deacetylation and subsequent N-sulfation of selected GlcNAc residues of the [GlcUA-GlcNAc] polymer | adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
C57BL/6 mice, isozymes NDST-1-4 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bone marrow cell | NDST-2 correlates strongly with mast cell maturation, whereas the expression of the NDST-1 isoform is approximately equal at all stages of maturation. NDST-2 correlates strongly with mast cell maturation, whereas the expression of the NDST-1 isoform is approximately equal at all stages of maturation. NDST-1 expression is detected already in the early stage, day 0, bone marrow cells, but the level of expression does not increase throughout the process of mast cell maturation. NDST-2 expression follows an entirely different pattern, with low expression in day 0 and day 7 cultures, followed by a markedly increased level of expression starting from day 14 and has a continuously increased expression throughout the entire culture period. NDST-2 is downregulated after mast cell activation | Mus musculus | - |
| mast cell | NDST-2 correlates strongly with mast cell maturation, whereas the expression of the NDST-1 isoform is approximately equal at all stages of maturation. NDST-1 expression is detected already in the early stage, day 0, bone marrow cells, but the level of expression does not increase throughout the process of mast cell maturation. NDST-2 expression follows an entirely different pattern, with low expression in day 0 and day 7 cultures, followed by a markedly increased level of expression starting from day 14 and has a continuously increased expression throughout the entire culture period. NDST-2 is downregulated after mast cell activation | Mus musculus | - |
| additional information | isozymes NDST-1 and NDST-2 appear to be predominant in adult tissue | Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine | - |
Mus musculus | adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine | - |
? | |
| 3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine | the sulfation of heparin is initiated by the NDST enzymes, which catalyze the N-deacetylation and subsequent N-sulfation of selected GlcNAc residues of the [GlcUA-GlcNAc] polymer | Mus musculus | adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine | - |
? | |
| 3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine | - |
Mus musculus C57BL/6 | adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine | - |
? | |
| 3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine | the sulfation of heparin is initiated by the NDST enzymes, which catalyze the N-deacetylation and subsequent N-sulfation of selected GlcNAc residues of the [GlcUA-GlcNAc] polymer | Mus musculus C57BL/6 | adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| N-deacetylase/N-sulfotransferase-1 | - |
Mus musculus |
| N-deacetylase/N-sulfotransferase-2 | - |
Mus musculus |
| NDST-1 | - |
Mus musculus |
| NDST-2 | - |
Mus musculus |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Mus musculus | NDST-2 is downregulated after mast cell activation, e.g. by calcium ionophore or IgE ligation. Down-regulation of NDST-2 gene by A23187 | down |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | NDST-2 is the first and a key enzyme in heparin synthesis, with the following modifications being dependent on the reactions catalyzed by the NDSTs | Mus musculus |
| physiological function | NDSTs have key regulatory functions in determining the degree of heparin/heparan sulfate sulfation. NDST-2 has a role in the formation of fully sulfated heparin attached to the serglycin core protein | Mus musculus |
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