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Literature summary for 2.8.2.8 extracted from

  • Deligny, A.; Denys, A.; Marcant, A.; Melchior, A.; Mazurier, J.; van Kuppevelt, T.H.; Allain, F.
    Synthesis of heparan sulfate with cyclophilin B-binding properties is determined by cell type-specific expression of sulfotransferases (2010), J. Biol. Chem., 285, 1701-1715.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.8

Cloned(Commentary)

Cloned (Comment) Organism
isozymes NDST1 and NDST2 Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information lack of NDST1 affects heparan sulfate structure in all tissues tested, with a dramatic reduction in N- and O-sulfation of the polysaccharide. Silencing the expression of NDST1, NDST2, 2-OST, and 3-OST3 by RNA interference efficiently decreases binding and activity of cyclophilin B Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine Homo sapiens NDST1 is able to partially sulfate exogenous substrate in the absence of NDST2 but not vice versa adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
 ?
additional information Homo sapiens NDST1 and NDST2 have no redundant activities but rather have complementary activities in making N-sulfated heparan sulfate motifs with cyclophilin B-binding properties. Synthesis of heparan sulfate with cyclophilin B-binding properties is determined by cell type-specific expression of sulfotransferases ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 isozymes NDST1 and NDST2
-

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
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JURKAT cell clone E6-1, ATCC TIB-152 Homo sapiens
-
macrophage high expression level of isozyme NDST2 Homo sapiens
-
MCF-7 cell
-
 Homo sapiens
-
MDA-MB-231 cell
-
 Homo sapiens
-
monocyte high expression level of isozyme NDST2. The expression of NDST2 in monocytes is modified following cell activation or maturation, although the same treatment has no significant effect on the level of mRNAencoding NDST1 Homo sapiens
-
additional information NDST1 and NDST2 are widely expressed in all the tissues analyzed, overview. Synthesis of heparan sulfate with cyclophilin B-binding properties is determined by cell type-specific expression of sulfotransferases Homo sapiens
-
T-47D cell
-
 Homo sapiens
-
T-lymphocyte CD4+ cells, high expression level of isozyme NDST2. NDST1 is barely detected in naive T-lymphocytes by comparison with memory and activated CD4 T cells, whereas no notable variation in the expression of NDST2 can be distinguished between T cell subsets Homo sapiens
-
THP-1 cell promonocytic leukemia cells Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
-
 Homo sapiens adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
 ?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine NDST1 is able to partially sulfate exogenous substrate in the absence of NDST2 but not vice versa Homo sapiens adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
 ?
additional information NDST1 and NDST2 have no redundant activities but rather have complementary activities in making N-sulfated heparan sulfate motifs with cyclophilin B-binding properties. Synthesis of heparan sulfate with cyclophilin B-binding properties is determined by cell type-specific expression of sulfotransferases Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
N-deacetylase/N-sulfotransferase
-
 Homo sapiens
Ndst1
-
 Homo sapiens
NDST2
-
 Homo sapiens

General Information

General Information Comment Organism
metabolism in the general scheme of heparan sulfate biosynthesis, GlcNAc N-deacetylation and N-sulfation by NDSTs create the prerequisite substrate needed for the next enzymatic modifications Homo sapiens
physiological function NDST1 is required for initiation of N-sulfation of the nascent precursor, whereas NDST2 may fill in or extend the sections of N-sulfated residues in heparin and highly sulfated heparan sulfate species Homo sapiens