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Literature summary for 2.8.2.8 extracted from
- Molecular cloning and expression of a third member of the heparan sulfate/heparin GlcNAc N-deacetylase/N-sulfotransferase family (1999), J. Biol. Chem., 274, 2690-2695.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| brain cDNA library, DNA sequence determination and analysis | Homo sapiens |
| construction of a chimeric enzyme consisiting of a soluble enzyme form NDST3 N-terminally fused to a protein A fragment, expression in COS-7 cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | CHO cell mutant pgsE 606 with reduced N-sulfotransferase activity shows reduced expression of enzyme form NDST1, but normal expression level of enzyme form NDST2 | Cricetulus griseus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cricetulus griseus | - |
wild-type and mutant pgsE-606 | - |
| Cricetulus griseus | - |
enzyme form NDST1 and NDST2, but not NDST3 | - |
| Homo sapiens | O95803 | enzyme form NDST3, bifunctional enzyme possessing heparan sulfate N-deacetylase and N-sulfotransferase activity | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | fetal | Homo sapiens | - |
| cell culture | CHO cell | Cricetulus griseus | - |
| CHO cell | - |
Cricetulus griseus | - |
| fetus | brain | Homo sapiens | - |
| kidney | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| ovary | CHO cells | Cricetulus griseus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine | - |
Cricetulus griseus | adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine | - |
? |  |
| 3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine | - |
Homo sapiens | adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GlcNAc N-deacetylase/N-sulfotransferase | - |
Cricetulus griseus |
| GlcNAc N-deacetylase/N-sulfotransferase | - |
Homo sapiens |
| NDST | - |
Cricetulus griseus |
| NDST | - |
Homo sapiens |
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