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Literature summary for 2.8.2.8 extracted from
- 3-Phosphoadenylylsulfate:N-desulfoheparin sulfotransferase associated with a postmicrosomal particulate mastocytoma fraction (1967), Arch. Biochem. Biophys., 119, 387-397.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Mus musculus |  |
| PCMB | - |
Mus musculus | |
| phenylmercuric acetate | - |
Mus musculus | |
| Zn2+ | - |
Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | associated with postmicrosomal fraction | Mus musculus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates at 10 mM | Mus musculus | |
| additional information | greatest activity in presence of sodium phosphate buffer, in imidazole-HCl reaction rate is lower | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
Furth mouse | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mastocytoma cell | - |
Mus musculus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -18°C, stable for at least 4 months | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + chondroitin 4-sulfate | weak activity | Mus musculus | ? | - |
? | |
| 3'-phosphoadenylylsulfate + dermatan sulfate | weak activity | Mus musculus | ? | - |
? | |
| 3'-phosphoadenylylsulfate + heparan sulfate | - |
Mus musculus | adenosine 3',5'-bisphosphate + N-sulfoheparan sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + N-desulfated heparin | - |
Mus musculus | ? | - |
? | |
| additional information | p-nitrophenol is no acceptor | Mus musculus | ? | - |
? | |
| additional information | heparin is no acceptor | Mus musculus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
2 min, no effect | Mus musculus |
| 55 | - |
- |
Mus musculus |
| 70 | - |
- |
Mus musculus |
| 85 | - |
2 min, complete loss of activity | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | 7.2 | - |
Mus musculus |
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Release 2023.1 (January 2023)
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