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Literature summary for 2.8.2.8 extracted from

  • Cheung, W.F.; Eriksson, I.; Kusche-Gulberg, M.; Lindahl, U.; Kjellen, L.
    Expression of mouse mastocytoma glucosaminyl N-deacetylase/N-sulfotransferase in human kidney 293 cells results in increased N-sulfation of heparan sulfate (1996), Biochemistry, 35, 5250-5256.
    View publication on PubMed
Search for more literature for 2.8.2.8

Cloned(Commentary)

Cloned (Comment) Organism
stable functional overexpression in human kidney cell line 293, 2.5fold increase in N-sulfation and 8fold increase of N-deacetylation of glucosaminyl residues of human heparan sulfate in transfected cells Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine Mus musculus bifunctional enzyme initiates heparin and heparan sulfate biosynthesis adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
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 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
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 bifunctional enzyme possessing glucosaminyl N-deacetylase and N-sulfotransferase activity
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Source Tissue

Source Tissue Comment Organism Textmining
mastocytoma cell heparin producing cells Mus musculus
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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 bifunctional activity of different recombinant clones of transfected human kidney 293 cells Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine substrate needs to be deacetylated Mus musculus adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
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 ?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine recombinant bifunctional enzyme overexpressed in human kidney cells shows 2.5fold increase in N-sulfation and 8fold increase of N-deacetylation of glucosaminyl residues of human heparan sulfate in vivo Mus musculus adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
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 ?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine structural analysis of substrate and product Mus musculus adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
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 ?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine bifunctional enzyme initiates heparin and heparan sulfate biosynthesis Mus musculus adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
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 ?
3'-phosphoadenylylsulfate + heparin
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 Mus musculus adenosine 3',5'-bisphosphate + N-sulfoheparin
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 ?

Synonyms

Synonyms Comment Organism
glucosaminyl N-deacetylase/N-sulfotransferase
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
 assay at Mus musculus