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Literature summary for 2.8.2.6 extracted from
- Choline sulphokinase (sulfotransferase) (1964), J. Biochem., 56, 81-91.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ethanol | - |
Aspergillus nidulans |  |
| glycol | - |
Aspergillus nidulans | |
| n-Propanol | maximal activation, at 2.5 mM, inhibition above 25 mM | Aspergillus nidulans | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-Naphthylamine | - |
Aspergillus nidulans | |
| 2,4-dinitrophenol | - |
Aspergillus nidulans | |
| 3-Aminophenol | - |
Aspergillus nidulans | |
| 4-Dimethylaminophenol | - |
Aspergillus nidulans | |
| 4-nitrophenol | competitive inhibition | Aspergillus nidulans | |
| Acetylcholine | - |
Aspergillus nidulans | |
| beta-homocholine | - |
Aspergillus nidulans | |
| carnitine | competitive inhibition | Aspergillus nidulans | |
| Co2+ | sensitive to metal ions, almost complete inhibition at 6.0 mM | Aspergillus nidulans | |
| cyanide | - |
Aspergillus nidulans | |
| diethanolamine | - |
Aspergillus nidulans | |
| Dimethylaminoethanethiol | - |
Aspergillus nidulans | |
| dimethylaminoethanol | substrate inhibition | Aspergillus nidulans | |
| Dimethylaminopropan-1-ol | competitive inhibition | Aspergillus nidulans | |
| Dimethylaminopropen-1-ol | - |
Aspergillus nidulans | |
| dimethylethylaminoethanol | substrate inhibition | Aspergillus nidulans | |
| ethyltrimethylammonium bromide | - |
Aspergillus nidulans | |
| Fe3+ | sensitive to metal ions, almost complete inhibition at 6.0 mM | Aspergillus nidulans | |
| gamma-homocholine | - |
Aspergillus nidulans | |
| hexadecyltrimethylammonium bromide | - |
Aspergillus nidulans | |
| Mn2+ | sensitive to metal ions, almost complete inhibition at 6.0 mM | Aspergillus nidulans | |
| additional information | iodoacetate is less effective as inhibitor, di-isopropylfluorophosphate is without action | Aspergillus nidulans | |
| N-ethylmaleimide | - |
Aspergillus nidulans | |
| n-Propanol | concentrations above 0.025 M | Aspergillus nidulans | |
| Ni2+ | sensitive to metal ions, almost complete inhibition at 6.0 mM | Aspergillus nidulans | |
| p-chloromercuribenzoate | - |
Aspergillus nidulans | |
| phenol | strong inhibitor | Aspergillus nidulans | |
| Prostigmine | - |
Aspergillus nidulans | |
| sulfate | - |
Aspergillus nidulans | |
| thiocholine | - |
Aspergillus nidulans | |
| triethanolamine | - |
Aspergillus nidulans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0222 | - |
3'-phosphoadenylylsulfate | pH 7.3, 37°C | Aspergillus nidulans | |
| 12 | - |
choline | pH 7.3, 37°C | Aspergillus nidulans | |
| 20 | - |
N,N-dimethylethylaminoethanol | pH 7.3, 37°C | Aspergillus nidulans | |
| 25 | - |
dimethylaminoethanol | pH 7.3, 37°C | Aspergillus nidulans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not even a partial requirement for Mg2+ | Aspergillus nidulans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + choline | Aspergillus nidulans | - |
adenosine 3',5'-bisphosphate + choline sulfate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus nidulans | - |
CMI 38,595 S eta, sulfateless mutant | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Aspergillus nidulans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, freezing and storing for 3 days results in complete loss of activity | Aspergillus nidulans |
| 2°C, more stable after (NH4)2SO4 or acetone fractionation, activity is reduced by 85% during 4 weeks | Aspergillus nidulans |
| 2°C, very unstable, loses 95% of its activity after 5 days | Aspergillus nidulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + choline | - |
Aspergillus nidulans | adenosine 3',5'-bisphosphate + choline sulfate | - |
ir | |
| 3'-phosphoadenylylsulfate + choline | sulfate transfer appears to be irreversible in vitro | Aspergillus nidulans | adenosine 3',5'-bisphosphate + choline sulfate | - |
ir | |
| 3'-phosphoadenylylsulfate + N,N-dimethylaminoethanol | - |
Aspergillus nidulans | adenosine 3',5'-bisphosphate + N,N-dimethylaminoethanol O-sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + N,N-dimethylethylaminoethanol | - |
Aspergillus nidulans | adenosine 3',5'-bisphosphate + N,N-dimethylethylaminoethanol O-sulfate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 30 | - |
Aspergillus nidulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 46 | - |
no loss of activity during 10 min at pH 9.2, 30% loss at pH 7.3, 34% loss at pH 5.0 | Aspergillus nidulans |
| 55 | - |
almost completely inactivated | Aspergillus nidulans |
| 58.5 | - |
complete loss of activity at pH 5.0, 85% activity retained at pH 9.2 | Aspergillus nidulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Aspergillus nidulans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | about 50% of activity maximum at pH 5.8 and 10.1 | Aspergillus nidulans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 12 | stable at room temperature in the pH range 8.0-11.0, above pH 11.0 enzyme is rapidly inactivated, 80% inactivation at pH 12.0, below pH 8.0 stablity declines slowly with decreasing pH, maximum stability at pH 8,5-10.5 | Aspergillus nidulans |
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Release 2023.1 (January 2023)
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