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Literature summary for 2.8.2.5 extracted from

  • Zhou, Z.; Li, Q.; Huang, H.; Wang, H.; Wang, Y.; Du, G.; Chen, J.; Kang, Z.
    A microbial-enzymatic strategy for producing chondroitin sulfate glycosaminoglycans (2018), Biotechnol. Bioeng., 115, 1561-1570 .
    View publication on PubMed
Search for more literature for 2.8.2.5

Application

Application Comment Organism
synthesis the enzyme is used for large scale chondroitin sulfate synthesis in a coupled system with aryl sulfotransferase IV and chondroitin 6-sulfotransferase, overview Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
gene C4ST, recombinant expression in Bacillus subtilis strain E168C, Escherichia coli strain BL21(DE3), and Pichia pastoris strain GS115, coexpression with ASSTIV and C6ST from Rattus norvegicus, subcloning in Escherichia coli strain JM109. Chondroitin extraction and quantification Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information reconstitution of the chondroitin biosynthesis pathway in a recombinant Bacillus subtilis strain using sucrose as carbon source, method optimization, overview. Specific sulfation transformation systems are successfully constructed and optimized by combining the purified aryl sulfotransferase IV (ASST IV, EC 2.8.2.1), chondroitin 4-sulfotransferase (C4ST) and chondroitin 6-sulfotransferase (C6ST, EC 2.8.2.17). Chondroitin sulfate A and C are enzymatically transformed from chondroitin at conversion rates of 98% and 96%, respectively. The ASST IV enzyme is used for PAPS regeneration. Purification of CS disaccharides by anion exchange chrmatography and NMR analysis of the composition of CS disaccharides Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl sulfate + chondroitin Rattus norvegicus
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 adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P69478
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + chondroitin
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
-
 ?

Synonyms

Synonyms Comment Organism
C4ST
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Rattus norvegicus