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Literature summary for 2.8.2.5 extracted from
- Sulfation of chondroitin oligosaccharides in vitro. Analysis of sulfation ratios (1985), J. Biol. Chem., 260, 14446-14451.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | stimulation only at pH 6.0 | Gallus gallus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-(N-Morpholino)ethanesulfonic acid buffer | pH above 6.0 | Gallus gallus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Gallus gallus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Gallus gallus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | stimulates, to a lesser intent than Mn2+ | Gallus gallus | |
| Mn2+ | stimulates | Gallus gallus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + chondroitin | Gallus gallus | - |
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | chondrocytes | Gallus gallus | - |
| chondrocyte | embryo | Gallus gallus | - |
| chondrocyte | cell culture | Gallus gallus | - |
| embryo | cell culture | Gallus gallus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + chondroitin | - |
Gallus gallus | adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + chondroitin-derived oligosaccharides | - |
Gallus gallus | adenosine 3',5'-bisphosphate + mixture of 4'-monosulfated oligosaccharides | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | - |
Gallus gallus |
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