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Literature summary for 2.8.2.4 extracted from

  • Adams, J.B.; Ellyard, R.K.; Low, J.
    Enzymic synthesis of steroid sulphates. IX. Physical and chemical properties of purified oestrogen sulphotransferase from bovine adrenal glands, the nature of its isoenzymic forms and a proposed model to explain its wave-like kinetics (1974), Biochim. Biophys. Acta, 370, 160-188.
    View publication on PubMed
Search for more literature for 2.8.2.4

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activation Bos taurus
cysteine
-
 Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
3'-AMP
-
 Bos taurus
3'-phosphoadenylylsulfate competitive inhibition Bos taurus
ADP non-competitive inhibitor Bos taurus
ATP mixed-type inhibition Bos taurus
p-hydroxymercuribenzoate
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 estrone pH 8.1, 37°C Bos taurus
0.037
-
 3'-phosphoadenylylsulfate pH 8.1, 37°C Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73800
-
 sedimentation equilibrium centrifugation Bos taurus
74000
-
 sucrose density gradient, pH 5.8 Bos taurus
74800
-
 meniscus depletion method, sucrose density gradient centrifugation, pH 7.0 Bos taurus
76000
-
 sucrose density gradient centrifugation, pH 9.0 Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + estrone Bos taurus
-
 adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
 r

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
adrenal enzyme Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + 17beta-estradiol
-
 Bos taurus adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
 r
3'-phosphoadenylylsulfate + estrone
-
 Bos taurus adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 74000, SDS-PAGE Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.056
-
 3'-phosphoadenylylsulfate pH 8.1, 37°C Bos taurus
1.9
-
 3'-AMP pH 8.1, 37°C Bos taurus
2.8
-
 ADP pH 8.1, 37°C Bos taurus