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Literature summary for 2.8.2.38 extracted from

  • Luczak, S.; Forlani, F.; Papenbrock, J.
    Desulfo-glucosinolate sulfotransferases isolated from several Arabidopsis thaliana ecotypes differ in their sequence and enzyme kinetics (2013), Plant Physiol. Biochem., 63, 15-23.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli XL1-Blue cells Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
glucoerucin in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C Arabidopsis thaliana
0.055
-
glucoiberverin in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C Arabidopsis thaliana
0.058
-
glucoerucin in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C Arabidopsis thaliana
0.06
-
glucoerucin in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C Arabidopsis thaliana
0.06
-
glucoiberverin in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C Arabidopsis thaliana
0.067
-
glucoiberverin in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C Arabidopsis thaliana
0.081
-
glucoerucin in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C Arabidopsis thaliana
0.09
-
glucoiberverin in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C Arabidopsis thaliana
0.096
-
glucoerucin in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C Arabidopsis thaliana
0.1
-
glucoiberverin in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C Arabidopsis thaliana
0.13
-
glucoerucin in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana ecotype Bla-10
-
-
-
Arabidopsis thaliana ecotype C24
-
-
-
Arabidopsis thaliana ecotype Col-0
-
-
-
Arabidopsis thaliana ecotype Cvi-0
-
-
-
Arabidopsis thaliana ecotype Ler-1
-
-
-
Arabidopsis thaliana ecotype Ws
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine 3',5'-bisphosphate + glucoerucin
-
Arabidopsis thaliana ?
-
?
adenosine 3',5'-bisphosphate + glucoerucin
-
Arabidopsis thaliana ecotype C24 ?
-
?
adenosine 3',5'-bisphosphate + glucoerucin
-
Arabidopsis thaliana ecotype Cvi-0 ?
-
?
adenosine 3',5'-bisphosphate + glucoerucin
-
Arabidopsis thaliana ecotype Col-0 ?
-
?
adenosine 3',5'-bisphosphate + glucoerucin
-
Arabidopsis thaliana ecotype Ws ?
-
?
adenosine 3',5'-bisphosphate + glucoerucin
-
Arabidopsis thaliana ecotype Bla-10 ?
-
?
adenosine 3',5'-bisphosphate + glucoerucin
-
Arabidopsis thaliana ecotype Ler-1 ?
-
?
adenosine 3',5'-bisphosphate + glucoiberverin
-
Arabidopsis thaliana ?
-
?
adenosine 3',5'-bisphosphate + glucoiberverin
-
Arabidopsis thaliana ecotype C24 ?
-
?
adenosine 3',5'-bisphosphate + glucoiberverin
-
Arabidopsis thaliana ecotype Cvi-0 ?
-
?
adenosine 3',5'-bisphosphate + glucoiberverin
-
Arabidopsis thaliana ecotype Col-0 ?
-
?
adenosine 3',5'-bisphosphate + glucoiberverin
-
Arabidopsis thaliana ecotype Ws ?
-
?
adenosine 3',5'-bisphosphate + glucoiberverin
-
Arabidopsis thaliana ecotype Bla-10 ?
-
?
adenosine 3',5'-bisphosphate + glucoiberverin
-
Arabidopsis thaliana ecotype Ler-1 ?
-
?

Synonyms

Synonyms Comment Organism
desulfo-glucosinolate sulfotransferase
-
Arabidopsis thaliana
SOT18
-
Arabidopsis thaliana