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Literature summary for 2.8.2.38 extracted from
- Kinetics and substrate specificities of desulfo-glucosinolate sulfotransferases in Arabidopsis thaliana (2009), Physiol. Plant., 135, 140-149.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of isozyme SOT16 from ecotype C24 in Escherichia coli | Arabidopsis thaliana |
| overexpression of isozymes SOT17 from ecotype C24 in Escherichia coli | Arabidopsis thaliana |
| overexpression of the isozymes SOT18 from ecotype C24 and ecotype Columbia-0 in Escherichia coli | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics, overview | Arabidopsis thaliana | |
| 0.043 | - |
desulfo-4-methylthiobutylglucosinolate | pH 9.0, 37°C, isozyme SOT18, ecotype Columbia-0 | Arabidopsis thaliana |  |
| 0.055 | - |
desulfo-3-methylthiopropylglucosinolate | pH 9.0, 37°C, isozyme SOT18, ecotype Columbia-0 | Arabidopsis thaliana | |
| 0.065 | - |
desulfo-4-methylthiobutylglucosinolate | pH 9.0, 37°C, isozyme SOT17 | Arabidopsis thaliana | |
| 0.07 | - |
desulfo-3-methylthiopropylglucosinolate | pH 9.0, 37°C, isozyme SOT16 | Arabidopsis thaliana | |
| 0.08 | - |
desulfo-4-methylthiobutylglucosinolate | pH 9.0, 37°C, isozyme SOT16 | Arabidopsis thaliana | |
| 0.088 | - |
desulfo-3-methylthiopropylglucosinolate | pH 9.0, 37°C, isozyme SOT17 | Arabidopsis thaliana | |
| 0.1 | - |
desulfo-3-methylthiopropylglucosinolate | pH 9.0, 37°C, isozyme SOT18, ecotype C24 | Arabidopsis thaliana | |
| 0.13 | - |
desulfo-4-methylthiobutylglucosinolate | pH 9.0, 37°C, isozyme SOT18, ecotype C24 | Arabidopsis thaliana | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Arabidopsis thaliana | qualitative glucosinolate analysis in leaves of ecotype C24, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9C9C9 | isozyme SOT18; ecotypes C24 and Columbia-0, isozyme SOT18 | - |
| Arabidopsis thaliana | Q9C9D0 | isozyme SOT16; ecotype C24, isozyme SOT16 | - |
| Arabidopsis thaliana | Q9FZ80 | isozyme SOT17; ecotype C24, isozyme SOT17 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant isozyme SOT16 from Escherichia coli by affinity chromatography | Arabidopsis thaliana |
| recombinant isozyme SOT17 from Escherichia coli by affinity chromatography | Arabidopsis thaliana |
| recombinant isozymes SOT18 from Escherichia coli by affinity chromatography | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + desulfo-3-methylthiopropyl glucosinolate | - |
Arabidopsis thaliana | adenosine-3',5'-bisphosphate + 3-methylthiopropyl glucosinolate | - |
? | |
| 3'-phosphoadenylyl sulfate + desulfo-4-methylthiobutyl glucosinolate | - |
Arabidopsis thaliana | adenosine-3',5'-bisphosphate + 4-methylthiobutyl glucosinolate | - |
? | |
| 3'-phosphoadenylyl sulfate + desulfo-7-methylthioheptyl glucosinolate | - |
Arabidopsis thaliana | adenosine-3',5'-bisphosphate + 7-methylthioheptyl glucosinolate | - |
? | |
| 3'-phosphoadenylyl sulfate + desulfo-7-methylthioheptyl glucosinolate | preferred substrate of isozyme SOT18 of ecotype C24 | Arabidopsis thaliana | adenosine-3',5'-bisphosphate + 7-methylthioheptyl glucosinolate | - |
? | |
| 3'-phosphoadenylyl sulfate + desulfo-8-methylthiooctyl glucosinolate | - |
Arabidopsis thaliana | adenosine-3',5'-bisphosphate + 8-methylthiooctyl glucosinolate | - |
? | |
| 3'-phosphoadenylyl sulfate + desulfo-8-methylthiooctyl glucosinolate | preferred substrate of isozyme SOT18 of ecotype C24 | Arabidopsis thaliana | adenosine-3',5'-bisphosphate + 8-methylthiooctyl glucosinolate | - |
? | |
| 3'-phosphoadenylyl sulfate + desulfo-indol-3-yl-methyl glucosinolate | - |
Arabidopsis thaliana | adenosine-3',5'-bisphosphate + indol-3-yl-methyl glucosinolate | - |
? | |
| 3'-phosphoadenylyl sulfate + desulfo-indol-3-yl-methyl glucosinolate | preferred substrate of isozyme SOT16 of ecotype C24 | Arabidopsis thaliana | adenosine-3',5'-bisphosphate + indol-3-yl-methyl glucosinolate | - |
? | |
| additional information | qualitative glucosinolate analysis in leaves of ecotype C24, overview | Arabidopsis thaliana | ? | - |
? | |
| additional information | ecotype C24 isozyme SOT18 prefers the long-chain desulfoglucosinolates, 7-methylthioheptyl and 8-methylthiooctyl, derived from methionine. The substrate specificty of the isozyme SOT18 from ecotype Columbia-0 differs from the SOT18 from ecotype C24, detailed overview. Substrate synthesis and optimization of the enzyme assay method, overview | Arabidopsis thaliana | ? | - |
? | |
| additional information | ecotype C24 isozyme substrate specificities: isozyme SOT17 shows an increased specific activity with increasing chain length of desulfoglucosinolates derived from methionine. Substrate synthesis and optimization of the enzyme assay method, overview | Arabidopsis thaliana | ? | - |
? | |
| additional information | ecotype C24 isozyme substrate specificities: preference of isozyme SOT16 for the indolic desulfoglucosinolate indol-3-yl-methylglucosinolate. Substrate synthesis and optimization of the enzyme assay method, overview | Arabidopsis thaliana | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| desulfo-Gl SOT | - |
Arabidopsis thaliana |
| desulfo-glucosinolate sulfotransferase | - |
Arabidopsis thaliana |
| ds-Gl SOT | - |
Arabidopsis thaliana |
| SOT | - |
Arabidopsis thaliana |
| SOT16 | - |
Arabidopsis thaliana |
| SOT17 | - |
Arabidopsis thaliana |
| SOT18 | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Arabidopsis thaliana |
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