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Literature summary for 2.8.2.36 extracted from

  • Shi, R.; Lamb, S.S.; Bhat, S.; Sulea, T.; Wright, G.D.; Matte, A.; Cygler, M.
    Crystal structure of StaL, a glycopeptide antibiotic sulfotransferase from Streptomyces toyocaensis (2007), J. Biol. Chem., 282, 13073-13086.
    View publication on PubMed
Search for more literature for 2.8.2.36

Cloned(Commentary)

Cloned (Comment) Organism
staL gene from Streptomyces toyocaensis is cloned into a modified pET28a vector and expressed in Escherichia coli BL21 (DE3) Streptomyces toyocaensis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method at 21°C, crystal structure of StaL and its complex with the cofactor product adenosine 3',5'-bisphosphate Streptomyces toyocaensis

Protein Variants

Protein Variants Comment Organism
E205A activity of the mutant enzyme is 4% compared to activity of wild-type enzyme Streptomyces toyocaensis
E206A activity of the mutant enzyme is 70% compared to activity of wild-type enzyme Streptomyces toyocaensis
F77E activity of the mutant enzyme is 8% compared to activity of wild-type enzyme Streptomyces toyocaensis
H43A activity of the mutant enzyme is 18% compared to activity of wild-type enzyme Streptomyces toyocaensis
H67A mutant does not express well and is not tested Streptomyces toyocaensis
L48E mutant does not express well and is not tested Streptomyces toyocaensis
L48K mutant does not express well and is not tested Streptomyces toyocaensis
R101A activity of the mutant enzyme is 92% compared to activity of wild-type enzyme Streptomyces toyocaensis
R202A activity of the mutant enzyme is 85% compared to activity of wild-type enzyme Streptomyces toyocaensis
S98A activity of the mutant enzyme is 40% compared to activity of wild-type enzyme Streptomyces toyocaensis
W132F activity of the mutant enzyme is 5% compared to activity of wild-type enzyme Streptomyces toyocaensis
W34F activity of the mutant enzyme is 4% compared to activity of wild-type enzyme Streptomyces toyocaensis

Organism

Organism UniProt Comment Textmining
Streptomyces toyocaensis Q8KLM3
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Streptomyces toyocaensis NRRL15009 Q8KLM3
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Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces toyocaensis