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Literature summary for 2.8.2.35 extracted from

  • Evers, M.R.; Xia, G.; Kang, H.G.; Schachner, M.; Baenziger, J.U.
    Molecular cloning and characterization of a dermatan-specific N-acetylgalactosamine 4-O-sulfotransferase (2001), J. Biol. Chem., 276, 36344-36353.
    View publication on PubMed
Search for more literature for 2.8.2.35

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phospho-5'-adenylyl sulfate + [dermatan]-N-acetylgalactosamine Homo sapiens
-
 adenosine 3'-5'-bisphosphate + [dermatan]-4-O-sulfo-N-acetylgalactosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8NCH0
-
-

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Homo sapiens
-
kidney low levels Homo sapiens
-
liver
-
 Homo sapiens
-
lung fetal, most highly expressed Homo sapiens
-
pancreas
-
 Homo sapiens
-
pituitary gland most highly expressed Homo sapiens
-
placenta most highly expressed Homo sapiens
-
skeletal muscle low levels Homo sapiens
-
SW-480 cell most highly expressed Homo sapiens
-
thyroid most highly expressed Homo sapiens
-
uterus most highly expressed Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phospho-5'-adenylyl sulfate + [dermatan]-N-acetylgalactosamine
-
 Homo sapiens adenosine 3'-5'-bisphosphate + [dermatan]-4-O-sulfo-N-acetylgalactosamine
-
 ?
3'-phospho-5'-adenylyl sulfate + [dermatan]-N-acetylgalactosamine desulfated dermatan sulfate from porcine intestine. The sulfation takes place at the 4-position of N-acetylgalactosamine residues of dermatan. D4ST-1 transfers sulfate to the C-4 hydroxyl of (1,4)-linked GalNAc that is substituted with an alpha-linked iduronic acid at the C-3 hydroxyl. D4ST-1 shows a strong preference in vitro for sulfate transfer to IdoUAalpha(1,3)GalNAcbeta(1,4) that is flanked by GlcUAbeta(1,3)GalNAcbeta(1,4) as compared with IdoUAalpha(1,3)GalNAcbeta(1,4) flanked by IdoUAalpha1,3GalNAcbeta1,4. The specificity of D4ST-1 when assayed in vitro suggests that the addition of sulfate to GalNAc occurs immediately after epimerization of GlcUA to IdoUA. While D4ST-1 is able to transfer sulfate to both dermatan and chondroitin, the rate of transfer is nearly 10fold greater to dermatan than to chondroitin when the same substrate concentrations are used. D4ST-1 only displays a significant transfer of sulfate to chondroitin at relatively high substrate concentrations, raising the possibility that the chondroitin is contaminated with small amounts of dermatan and/or that occasional iduronic acid residues are present in the chondroitin and utilized by the D4ST-1 Homo sapiens adenosine 3'-5'-bisphosphate + [dermatan]-4-O-sulfo-N-acetylgalactosamine
-
 ?

Synonyms

Synonyms Comment Organism
D4ST-1
-
 Homo sapiens
dermatan-4-sulfotransferase 1
-
 Homo sapiens
dermatan-4-sulfotransferase-1
-
 Homo sapiens
dermatan-specific N-acetylgalactosamine 4-O-sulfotransferase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Homo sapiens