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Literature summary for 2.8.2.33 extracted from

  • Yamaguchi, T.; Ohtake, S.; Kimata, K.; Habuchi, O.
    Molecular cloning of squid N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase and synthesis of a unique chondroitin sulfate containing E-D hybrid tetrasaccharide structure by the recombinant enzyme (2007), Glycobiology, 17, 1362-1376.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of FLAG-tagged GalNAc4S-6ST in COS-7 cells Todarodes pacificus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Todarodes pacificus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl sulfate + chondroitin A Todarodes pacificus synthesis of chondroitin sulfate E adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate E
-
?

Organism

Organism UniProt Comment Textmining
Todarodes pacificus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant FLAG-tagged GalNAc4S-6ST from COS-7 cells by anti-FLAG immunoaffinity chromatography Todarodes pacificus

Source Tissue

Source Tissue Comment Organism Textmining
cartilage
-
Todarodes pacificus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + chondroitin A synthesis of chondroitin sulfate E Todarodes pacificus adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate E
-
?
3'-phosphoadenylyl sulfate + chondroitin C synthesis of a unique chondroitin sulfate containing E-D hybrid tetrasaccharide structure by the recombinant enzyme Todarodes pacificus adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate ED
-
?

Synonyms

Synonyms Comment Organism
GalNAc4S-6ST
-
Todarodes pacificus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at Todarodes pacificus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at Todarodes pacificus