Any feedback?
Literature summary for 2.8.2.32 extracted from
- 5beta-Scymnol sulfotransferases from the liver of two Australian ray species (1998), Comp. Biochem. Physiol. B, 121B, 341-348.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenosine-3',5'-diphosphate | 0.1 mM, 95% inhibition | Trygonoptera sp. |  |
| adenosine-3',5'-diphosphate | 0.1 mM, 96% inhibition | Trygonorrhina fasciata | |
| iodoacetic acid | 0.1 mM, 16% inhibition | Trygonoptera sp. | |
| iodoacetic acid | 0.1 mM, 40% inhibition | Trygonorrhina fasciata | |
| NaN3 | 0.1 mM, 24% inhibition | Trygonoptera sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0059 | - |
(24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol | - |
Trygonorrhina fasciata | |
| 0.018 | - |
(24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5-beta-cholestane-27-ol | - |
Trygonoptera sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Trygonorrhina fasciata | 5829 | - |
| cytosol | - |
Trygonoptera sp. | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 0.5 mM, 35% activation | Trygonorrhina fasciata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
gel filtration | Trygonorrhina fasciata |
| 69000 | - |
gel filtration | Trygonoptera sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol | Trygonorrhina fasciata | trivial name 5beta-scymnol | adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-sulfate | trivial name 5beta-scymnol sulfate | ? | |
| 3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol | Trygonoptera sp. | trivial name 5beta-scymnol | adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-sulfate | trivial name 5beta-scymnol sulfate | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trygonoptera sp. | - |
- |
- |
| Trygonorrhina fasciata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| hydroxylapatite, DEAE-Sephacel | Trygonorrhina fasciata |
| Sephadex G-100, hydroxylapatite | Trygonoptera sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Trygonorrhina fasciata | - |
| kidney | - |
Trygonoptera sp. | - |
| liver | - |
Trygonorrhina fasciata | - |
| liver | - |
Trygonoptera sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0000026 | - |
- |
Trygonoptera sp. |
| 0.0000035 | - |
- |
Trygonorrhina fasciata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5-beta-cholestane-27-yl | trivial name 5beta-scymnol | Trygonorrhina fasciata | adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-yl sulfate | trivial name 5beta-scymnol sulfate | ? | |
| 3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol | trivial name 5beta-scymnol | Trygonorrhina fasciata | adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-sulfate | trivial name 5beta-scymnol sulfate | ? | |
| 3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol | trivial name 5beta-scymnol | Trygonoptera sp. | adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-sulfate | trivial name 5beta-scymnol sulfate | ? | |
| 3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol | trivial name 5beta-scymnol | Trygonoptera sp. | adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-sulfate | trivial name beta-scymnol sulfate | ? | |
| 3'-phosphoadenosine 5'-phosphosulfate + 5alpha-cyprinol | 174% of activity with 5beta-scymnol | Trygonorrhina fasciata | adenosine 3',5'-diphosphate + 5alpha-cyprinol sulfate | - |
? | |
| 3'-phosphoadenosine 5'-phosphosulfate + 5alpha-cyprinol | 250% of activity with 5beta-scymnol | Trygonoptera sp. | adenosine 3',5'-diphosphate + 5alpha-cyprinol sulfate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Trygonorrhina fasciata |
| 30 | - |
- |
Trygonoptera sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | 37 | - |
Trygonoptera sp. |
| 25 | 45 | - |
Trygonorrhina fasciata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Trygonorrhina fasciata |
| 6.5 | - |
- |
Trygonoptera sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | - |
Trygonorrhina fasciata |
| 5.5 | 7.5 | - |
Trygonoptera sp. |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00098 | - |
adenosine-3',5'-diphosphate | - |
Trygonoptera sp. | |
| 0.019 | - |
adenosine-3',5'-diphosphate | - |
Trygonorrhina fasciata | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
