Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine-3',5'-diphosphate | 0.1 mM, 95% inhibition | Trygonoptera sp. | |
adenosine-3',5'-diphosphate | 0.1 mM, 96% inhibition | Trygonorrhina fasciata | |
iodoacetic acid | 0.1 mM, 16% inhibition | Trygonoptera sp. | |
iodoacetic acid | 0.1 mM, 40% inhibition | Trygonorrhina fasciata | |
NaN3 | 0.1 mM, 24% inhibition | Trygonoptera sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0059 | - |
(24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol | - |
Trygonorrhina fasciata | |
0.018 | - |
(24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5-beta-cholestane-27-ol | - |
Trygonoptera sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Trygonorrhina fasciata | 5829 | - |
cytosol | - |
Trygonoptera sp. | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 0.5 mM, 35% activation | Trygonorrhina fasciata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
gel filtration | Trygonorrhina fasciata |
69000 | - |
gel filtration | Trygonoptera sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol | Trygonorrhina fasciata | trivial name 5beta-scymnol | adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-sulfate | trivial name 5beta-scymnol sulfate | ? | |
3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol | Trygonoptera sp. | trivial name 5beta-scymnol | adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-sulfate | trivial name 5beta-scymnol sulfate | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trygonoptera sp. | - |
- |
- |
Trygonorrhina fasciata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
hydroxylapatite, DEAE-Sephacel | Trygonorrhina fasciata |
Sephadex G-100, hydroxylapatite | Trygonoptera sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Trygonorrhina fasciata | - |
kidney | - |
Trygonoptera sp. | - |
liver | - |
Trygonorrhina fasciata | - |
liver | - |
Trygonoptera sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000026 | - |
- |
Trygonoptera sp. |
0.0000035 | - |
- |
Trygonorrhina fasciata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5-beta-cholestane-27-yl | trivial name 5beta-scymnol | Trygonorrhina fasciata | adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-yl sulfate | trivial name 5beta-scymnol sulfate | ? | |
3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol | trivial name 5beta-scymnol | Trygonorrhina fasciata | adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-sulfate | trivial name 5beta-scymnol sulfate | ? | |
3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol | trivial name 5beta-scymnol | Trygonoptera sp. | adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-sulfate | trivial name 5beta-scymnol sulfate | ? | |
3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol | trivial name 5beta-scymnol | Trygonoptera sp. | adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-sulfate | trivial name beta-scymnol sulfate | ? | |
3'-phosphoadenosine 5'-phosphosulfate + 5alpha-cyprinol | 174% of activity with 5beta-scymnol | Trygonorrhina fasciata | adenosine 3',5'-diphosphate + 5alpha-cyprinol sulfate | - |
? | |
3'-phosphoadenosine 5'-phosphosulfate + 5alpha-cyprinol | 250% of activity with 5beta-scymnol | Trygonoptera sp. | adenosine 3',5'-diphosphate + 5alpha-cyprinol sulfate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Trygonorrhina fasciata |
30 | - |
- |
Trygonoptera sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 37 | - |
Trygonoptera sp. |
25 | 45 | - |
Trygonorrhina fasciata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Trygonorrhina fasciata |
6.5 | - |
- |
Trygonoptera sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | - |
Trygonorrhina fasciata |
5.5 | 7.5 | - |
Trygonoptera sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00098 | - |
adenosine-3',5'-diphosphate | - |
Trygonoptera sp. | |
0.019 | - |
adenosine-3',5'-diphosphate | - |
Trygonorrhina fasciata |