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Literature summary for 2.8.2.25 extracted from

  • Varin, L.; Ibrahim, R.K.
    Partial purification and characterization of three flavonol-specific sulfotransferases from Flaveria chloraefolia (1989), Plant Physiol., 90, 977-981.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.25

Inhibitors

Inhibitors Comment Organism Structure
additional information EDTA, SH-reagents at 1 and 10 mM, e.g. p-chloromercuribenzoate, iodoacetate or iodoacetamide, are not inhibitory Flaveria chlorifolia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
 quercetin 30°C, pH 7.5 Flaveria chlorifolia
0.0002
-
 3'-phosphoadenylylsulfate cosubstrate quercetin, pH 7.5, 30°C Flaveria chlorifolia

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no divalent cation required Flaveria chlorifolia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 gel filtration Flaveria chlorifolia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + quercetin Flaveria chlorifolia
-
 adenosine 3',5'-bisphosphate + quercetin 3-sulfate
-
 ?
additional information Flaveria chlorifolia involved in biosynthesis of polysulfated flavonols ?
-
 ?

Organism

Organism UniProt Comment Textmining
Flaveria chlorifolia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Flaveria chlorifolia

Source Tissue

Source Tissue Comment Organism Textmining
shoot tip
-
 Flaveria chlorifolia
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00027
-
-
 Flaveria chlorifolia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + isorhamnetin 94% of activity compared to rhamnetin Flaveria chlorifolia adenosine 3',5'-bisphosphate + isorhamnetin 3-sulfate
-
 ?
3'-phosphoadenylylsulfate + kaempferol 48% of activity compared to rhamnetin Flaveria chlorifolia adenosine 3',5'-bisphosphate + kaempferol 3-sulfate
-
 ?
3'-phosphoadenylylsulfate + ombuin 37% of activity compared to rhamnetin Flaveria chlorifolia adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + patuletin 52% of activity compared to rhamnetin Flaveria chlorifolia adenosine 3',5'-bisphosphate + patuletin 3-sulfate
-
 ?
3'-phosphoadenylylsulfate + quercetin
-
 Flaveria chlorifolia adenosine 3',5'-bisphosphate + quercetin 3-sulfate
-
 ?
3'-phosphoadenylylsulfate + quercetin 58% of activity compared to rhamnetin Flaveria chlorifolia adenosine 3',5'-bisphosphate + quercetin 3-sulfate
-
 ?
3'-phosphoadenylylsulfate + rhamnetin best substrate Flaveria chlorifolia adenosine 3',5'-bisphosphate + rhamnetin 3-sulfate
-
 ?
3'-phosphoadenylylsulfate + tamarixetin 31% of activity compared to rhamnetin Flaveria chlorifolia adenosine 3',5'-bisphosphate + ?
-
 ?
additional information quercetagetin, gossypetin, myricetin or galangin are no substrates Flaveria chlorifolia ?
-
 ?
additional information involved in biosynthesis of polysulfated flavonols Flaveria chlorifolia ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Flaveria chlorifolia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 and pH 8.5, two maxima Flaveria chlorifolia
8.5
-
 and pH 6.5, two maxima Flaveria chlorifolia