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show all sequences of 2.8.2.24

Purification and properties of 3'-phosphoadenosine 5'-phosphosulfate: desulphoglucosinolate sulphotransefrase from Brassica juncea cell cultures

Jain, J.C.; Groot Wassink, J.W.D.; Kolenovsky, A.D.; Underhill, E.W.; Phytochemistry 29, 1425-1428 (1990)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
adenosine-3',5'-bisphosphate
competitive to 3'-phosphoadenylylsulfate, kinetics
Brassica juncea
Co2+
at low concentration
Brassica juncea
Cu2+
at low concentration
Brassica juncea
iodoacetamide
-
Brassica juncea
iodoacetic acid
weak inhibition
Brassica juncea
Iodosobenzoic acid
-
Brassica juncea
Mn2+
inhibitory at 10 mM, stimulating at 1 mM
Brassica juncea
additional information
no inhibition by 3'-AMP, 5'-AMP, 2',5'-AMP, 3',5'-cAMP, 5'-ADP, 5'-ATP, adenosine-5'-phosphosulfate, benzaldoxime, phenylacetohydroximate, phenylacetothiohydroximate, S-methylphenylacetothiohydroximate, benzylglucosinolate
Brassica juncea
N-Methylmaleimide
-
Brassica juncea
N-Pyrenylmaleimide
-
Brassica juncea
PCMB
-
Brassica juncea
Phenylmercuriacetate
-
Brassica juncea
Zn2+
at low concentration
Brassica juncea
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00078
-
3'-phosphoadenylylsulfate
pH 8.5-9.0, 30°C
Brassica juncea
0.0023
-
desulfobenzylglucosinolate
pH 8.5-9.0, 30°C
Brassica juncea
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
slight activation
Brassica juncea
Mn2+
23% activation at 1 mM, 10 mM inhibits by 53%
Brassica juncea
additional information
no absolute requirement for cations
Brassica juncea
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
gel filtration
Brassica juncea
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3'-phosphoadenylylsulfate + desulfobenzylglucosinolate
Brassica juncea
-
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
-
?
additional information
Brassica juncea
involved in the biosynthesis of glucosinolates
?
-
-
-
additional information
Brassica juncea
involved with EC 2.4.1.195 in final steps of thioglycoside biosynthesis in cruciferous plants
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Brassica juncea
-
cv. Cutlass
-
Purification (Commentary)
Commentary
Organism
partial, 230fold
Brassica juncea
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
derived from hypocotyls
Brassica juncea
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0000329
-
partially purified enzyme
Brassica juncea
Storage Stability
Storage Stability
Organism
-20°C, 20 mM Tris buffer, pH 7.5, 14 mM 2-mercaptoethanol, 10% glycerol, at least 2 months
Brassica juncea
4°C, 14 mM 2-mercaptoethanol, t1/2: about 2 weeks
Brassica juncea
4°C, more than 50% loss of activity within 48 h
Brassica juncea
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate
absolute specificity for desulfoglucosinolate structure
643825
Brassica juncea
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
643825
Brassica juncea
?
3'-phosphoadenylylsulfate + desulfobenzylglucosinolate
-
643825
Brassica juncea
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
-
-
?
additional information
no activity with adenosine-5'-phosphosulfate as donor
643825
Brassica juncea
?
-
-
-
-
additional information
involved in the biosynthesis of glucosinolates
643825
Brassica juncea
?
-
-
-
-
additional information
involved with EC 2.4.1.195 in final steps of thioglycoside biosynthesis in cruciferous plants
643825
Brassica juncea
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Brassica juncea
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
40
about 85% of maximal activity at 25°C and about half-maximal activity at 40°C
Brassica juncea
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
at least 1 h, inactivation above
Brassica juncea
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
9
maximal activity in Tris-HCl buffer
Brassica juncea
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0004
-
adenosine-3',5'-bisphosphate
pH 8.5-9.0, 30°C
Brassica juncea
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Brassica juncea
-
-
4.84
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
adenosine-3',5'-bisphosphate
competitive to 3'-phosphoadenylylsulfate, kinetics
Brassica juncea
Co2+
at low concentration
Brassica juncea
Cu2+
at low concentration
Brassica juncea
iodoacetamide
-
Brassica juncea
iodoacetic acid
weak inhibition
Brassica juncea
Iodosobenzoic acid
-
Brassica juncea
Mn2+
inhibitory at 10 mM, stimulating at 1 mM
Brassica juncea
additional information
no inhibition by 3'-AMP, 5'-AMP, 2',5'-AMP, 3',5'-cAMP, 5'-ADP, 5'-ATP, adenosine-5'-phosphosulfate, benzaldoxime, phenylacetohydroximate, phenylacetothiohydroximate, S-methylphenylacetothiohydroximate, benzylglucosinolate
Brassica juncea
N-Methylmaleimide
-
Brassica juncea
N-Pyrenylmaleimide
-
Brassica juncea
PCMB
-
Brassica juncea
Phenylmercuriacetate
-
Brassica juncea
Zn2+
at low concentration
Brassica juncea
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0004
-
adenosine-3',5'-bisphosphate
pH 8.5-9.0, 30°C
Brassica juncea
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00078
-
3'-phosphoadenylylsulfate
pH 8.5-9.0, 30°C
Brassica juncea
0.0023
-
desulfobenzylglucosinolate
pH 8.5-9.0, 30°C
Brassica juncea
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
slight activation
Brassica juncea
Mn2+
23% activation at 1 mM, 10 mM inhibits by 53%
Brassica juncea
additional information
no absolute requirement for cations
Brassica juncea
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
gel filtration
Brassica juncea
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3'-phosphoadenylylsulfate + desulfobenzylglucosinolate
Brassica juncea
-
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
-
?
additional information
Brassica juncea
involved in the biosynthesis of glucosinolates
?
-
-
-
additional information
Brassica juncea
involved with EC 2.4.1.195 in final steps of thioglycoside biosynthesis in cruciferous plants
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
partial, 230fold
Brassica juncea
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
derived from hypocotyls
Brassica juncea
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0000329
-
partially purified enzyme
Brassica juncea
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 20 mM Tris buffer, pH 7.5, 14 mM 2-mercaptoethanol, 10% glycerol, at least 2 months
Brassica juncea
4°C, 14 mM 2-mercaptoethanol, t1/2: about 2 weeks
Brassica juncea
4°C, more than 50% loss of activity within 48 h
Brassica juncea
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate
absolute specificity for desulfoglucosinolate structure
643825
Brassica juncea
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
643825
Brassica juncea
?
3'-phosphoadenylylsulfate + desulfobenzylglucosinolate
-
643825
Brassica juncea
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
-
-
?
additional information
no activity with adenosine-5'-phosphosulfate as donor
643825
Brassica juncea
?
-
-
-
-
additional information
involved in the biosynthesis of glucosinolates
643825
Brassica juncea
?
-
-
-
-
additional information
involved with EC 2.4.1.195 in final steps of thioglycoside biosynthesis in cruciferous plants
643825
Brassica juncea
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Brassica juncea
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
40
about 85% of maximal activity at 25°C and about half-maximal activity at 40°C
Brassica juncea
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
at least 1 h, inactivation above
Brassica juncea
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
9
maximal activity in Tris-HCl buffer
Brassica juncea
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Brassica juncea
-
-
4.84
Other publictions for EC 2.8.2.24
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
703650
Zang
Genome-wide identification of ...
Arabidopsis thaliana, Brassica rapa
FEBS J.
276
3559-3574
2009
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
661715
Klein
The three desulfoglucosinolate ...
Arabidopsis thaliana
FEBS J.
273
122-136
2006
-
-
-
-
2
-
1
7
1
-
3
-
-
1
-
-
1
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
7
1
-
3
-
-
-
-
1
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
662305
Piotrowski
Desulfoglucosinolate sulfotran ...
Arabidopsis thaliana
J. Biol. Chem.
279
50717-50725
2004
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643823
Jain
-
Persistent co-purification of ...
Brassica juncea
J. Plant Physiol.
136
356-361
1990
-
-
-
-
-
-
-
-
1
-
-
2
-
1
-
-
1
-
-
1
-
-
3
-
-
-
2
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
1
-
1
-
-
3
-
-
-
2
-
-
-
3
-
-
-
-
-
-
-
643824
Glendening
Partial purification and chara ...
Arabidopsis thaliana, Brassica rapa subsp. oleifera, Lepidium sativum, Sinapis alba, Tropaeolum majus
Plant Physiol.
94
811-816
1990
-
-
-
-
-
3
15
5
-
3
1
2
-
5
-
-
1
1
-
5
1
1
11
1
-
-
-
-
1
1
1
-
-
1
-
-
-
-
-
-
-
3
-
15
-
5
-
3
1
2
-
-
-
1
-
5
1
1
11
1
-
-
-
-
1
1
1
1
-
-
-
-
-
-
643825
Jain
-
Purification and properties of ...
Brassica juncea
Phytochemistry
29
1425-1428
1990
-
-
-
-
-
-
13
2
-
3
1
3
-
1
-
-
1
-
-
1
1
3
5
-
1
1
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
13
1
2
-
3
1
3
-
-
-
1
-
1
1
3
5
-
1
1
1
-
1
-
-
1
-
-
-
-
-
-
643821
Jain
A radioassay of enzymes cataly ...
Brassica juncea, Brassica napus, Brassica nigra, Brassica oleracea, Brassica rapa subsp. oleifera
Anal. Biochem.
178
137-140
1989
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
1
-
-
5
5
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
5
5
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643822
Jain
-
Distribution of enzymes cataly ...
Brassica juncea
Plant Sci.
64
25-29
1989
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-