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Literature summary for 2.8.2.23 extracted from

  • Moon, A.F.; Xu, Y.; Woody, S.M.; Krahn, J.M.; Linhardt, R.J.; Liu, J.; Pedersen, L.C.
    Dissecting the substrate recognition of 3-O-sulfotransferase for the biosynthesis of anticoagulant heparin (2012), Proc. Natl. Acad. Sci. USA, 109, 5265-5270.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3)-CodonPlusRIL cells Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
ternary complex of 3-OST-1/3’-phosphoadenosine 5’-phosphate with an acceptor heptasaccharide substrate, microbatch technique, using 23.6 mM Tris (pH 7.5), 142 mM NaCl, 0.1 M sodium citrate (pH 5.5) and 20% (w/v) PEG 3000 Mus musculus

Protein Variants

Protein Variants Comment Organism
H168A the mutant shows 51.1% activity compared to the wild type enzyme Mus musculus
H168E the mutant shows 19.1% activity compared to the wild type enzyme Mus musculus
K171A the mutant shows 83.9% activity compared to the wild type enzyme Mus musculus
N167A the mutant shows 40.4% activity compared to the wild type enzyme Mus musculus
R268A the mutant shows 7.2% activity compared to the wild type enzyme Mus musculus
V164E the mutant shows 20.3% activity compared to the wild type enzyme Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine Mus musculus
-
 adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus O35310
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose 4B resin column chromatography and Superdex 200 gel filtration Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
-
 Mus musculus adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
-
 ?

Synonyms

Synonyms Comment Organism
3-OST-1
-
 Mus musculus
heparan sulfate 3-O-sulfotransferase 1
-
 Mus musculus