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Literature summary for 2.8.2.23 extracted from

  • Edavettal, S.C.; Lee, K.A.; Negishi, M.; Linhardt, R.J.; Liu, J.; Pedersen, L.C.
    Crystal structure and mutational analysis of heparan sulfate 3-O-sulfotransferase isoform 1 (2004), J. Biol. Chem., 279, 25789-25797.
    View publication on PubMed
Search for more literature for 2.8.2.23

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21(DE3)RIL Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Mus musculus

Protein Variants

Protein Variants Comment Organism
D95A 0.0% of the wild type activity Mus musculus
D95N 8.1% of the wild type activity Mus musculus
E76A 30.7% of the wild type activity Mus musculus
E76Q 15.6% of the wild type activity Mus musculus
E88A 63.7% of the wild type activity Mus musculus
E90A 0.0% of the wild type activity Mus musculus
E90Q 0.0% of the wild type activity Mus musculus
E98A 65.6% of the wild type activity Mus musculus
H271A 109.0% of the wild type activity Mus musculus
H271F 42.0% of the wild type activity Mus musculus
H92A 0.0% of the wild type activity Mus musculus
H92F 0.0% of the wild type activity Mus musculus
K123A 0.2% of the wild type activity Mus musculus
K132A 49.1% of the wild type activity Mus musculus
K274A 17.4% of the wild type activity Mus musculus
K68A 0.1% of the wild type activity Mus musculus
N89A 69.5% of the wild type activity Mus musculus
N89D 74.4% of the wild type activity Mus musculus
Q163A 34.0% of the wild type activity Mus musculus
R197A 95.0% of the wild type activity Mus musculus
R276A 0.3% of the wild type activity Mus musculus
R67A 1.2% of the wild type activity Mus musculus
R67E 1.1% of the wild type activity Mus musculus
R72A 7.0% of the wild type activity Mus musculus
R72E 0.0% of the wild type activity Mus musculus
S273A 99.6% of the wild type activity Mus musculus
W96A 91.6% of the wild type activity Mus musculus
W96F 69.1% of the wild type activity Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 3'-phosphoadenosine 5'-phosphosulfate wild type Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenosine 5'-phosphosulfate + heparan sulfate-glucosamine Mus musculus regulation of blood coagulation heparan sulfate 3-O-sulfo glucosamine + adenosine 3',5'-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein from Escherichia coli Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenosine 5'-phosphosulfate + heparan sulfate-glucosamine
-
 Mus musculus heparan sulfate 3-O-sulfo glucosamine + adenosine 3',5'-bisphosphate
-
 ?
3'-phosphoadenosine 5'-phosphosulfate + heparan sulfate-glucosamine regulation of blood coagulation Mus musculus heparan sulfate 3-O-sulfo glucosamine + adenosine 3',5'-bisphosphate
-
 ?

Synonyms

Synonyms Comment Organism
3-OST-1
-
 Mus musculus
heparan sulfate 3-O-sulfotransferase isoform 1 Mus musculus