Literature summary for 2.8.2.22 extracted from

Grimshaw, J.P.; Stirnimann, C.U.; Brozzo, M.S.; Malojcic, G.; Gruetter, M.G.; Capitani, G.; Glockshuber, R.
DsbL and DsbI form a specific dithiol oxidase system for periplasmic arylsulfate sulfotransferase in uropathogenic Escherichia coli (2008), J. Mol. Biol., 380, 667-680.

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Activating Compound

Activating Compound	Comment	Organism	Structure
thioredoxin-like protein DsbL	besides the role of DsbL in the formation of the structural disulfide bond of ASST, it might improve the folding efficiency of ASST in vivo by preventing unspecific aggregation of ASST	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli K12	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
periplasm	-	Escherichia coli	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
128000	-	size-exclusion chromatography, homodimer	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
an aryl sulfate + a phenol	Escherichia coli	periplasmic ASST is an in vivo substrate of DsbL, the most reactive oxidizing thioredoxin-like protein known to date, ASST activity is detected only when both functional proteins DsbL and DsbI are coexpressed	a phenol + an aryl sulfate	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	Q8FDI4	virulent uropathogenic Escherichia coli strain CFT073, isolated from the blood of a patient with acute pyelonephritis, is cytotoxic for human renal cells	-

Purification (Commentary)

Purification (Comment)	Organism
chromatography	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
an aryl sulfate + a phenol	-	Escherichia coli	a phenol + an aryl sulfate	-	?
an aryl sulfate + a phenol	periplasmic ASST is an in vivo substrate of DsbL, the most reactive oxidizing thioredoxin-like protein known to date, ASST activity is detected only when both functional proteins DsbL and DsbI are coexpressed	Escherichia coli	a phenol + an aryl sulfate	-	?

Subunits

Subunits	Comment	Organism
homodimer	1 * 128000Da, size-exclusion chromatography	Escherichia coli

Synonyms

Synonyms	Comment	Organism
arylsulfate sulfotransferase	-	Escherichia coli
ASST	-	Escherichia coli

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Release 2023.1 (January 2023)