Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 33% inhibition at 2 mM | Klebsiella sp. | |
Cd2+ | almost complete inhibition at 2 mM | Klebsiella sp. | |
Cu2+ | 37% inhibition at 2 mM | Klebsiella sp. | |
EDTA | inactivation reversed by addition of metal ions | Klebsiella sp. | |
Ni2+ | 32% inhibition at 2 mM | Klebsiella sp. | |
Pb2+ | 35% inhibition at 2 mM | Klebsiella sp. | |
Zn2+ | 38% inhibition at 2 mM | Klebsiella sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
4-nitrophenyl sulfate | with phenol as second substrate, pH 8, 37°C | Klebsiella sp. | |
0.66 | - |
phenol | with 4-nitrophenyl sulfate as second substrate, pH 8, 37°C | Klebsiella sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 33% increase at 2 mM | Klebsiella sp. | |
Mg2+ | 65% increase at 2 mM | Klebsiella sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73000 | - |
2 * 73000, SDS-PAGE | Klebsiella sp. |
160000 | - |
gel filtration | Klebsiella sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Klebsiella sp. | may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation | ? | - |
? | |
additional information | Klebsiella sp. K-36 | may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella sp. | - |
K-36 | - |
Klebsiella sp. K-36 | - |
K-36 | - |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.82 | - |
- |
Klebsiella sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-acetylphenyl sulfate + phenol | 28% of the activity with 4-nitrophenyl sulfate | Klebsiella sp. | 4-acetylphenol + phenyl sulfate | - |
? | |
4-methylumbelliferyl sulfate + phenol | 55% of the activity with p-nitrophenyl sulfate | Klebsiella sp. | 4-methylumbelliferol + phenyl sulfate | - |
? | |
4-nitrophenyl sulfate + 3-chlorophenol | - |
Klebsiella sp. | 3-chlorophenyl sulfate + 4-nitrophenol | - |
? | |
4-nitrophenyl sulfate + 3-chlorophenol | - |
Klebsiella sp. K-36 | 3-chlorophenyl sulfate + 4-nitrophenol | - |
? | |
4-nitrophenyl sulfate + 9-phenanthrol | - |
Klebsiella sp. | 9-phenanthroyl sulfate + 4-nitrophenol | - |
? | |
4-nitrophenyl sulfate + 9-phenanthrol | - |
Klebsiella sp. K-36 | 9-phenanthroyl sulfate + 4-nitrophenol | - |
? | |
4-nitrophenyl sulfate + alpha-naphthol | - |
Klebsiella sp. | 4-nitrophenol + 1-naphththyl sulfate | - |
? | |
4-nitrophenyl sulfate + alpha-naphthol | - |
Klebsiella sp. K-36 | 4-nitrophenol + 1-naphththyl sulfate | - |
? | |
4-nitrophenyl sulfate + phenol | - |
Klebsiella sp. | phenyl sulfate + 4-nitrophenol | - |
? | |
estrone sulfate + phenol | 11% of the activity with p-nitrophenyl sulfate | Klebsiella sp. | estrone + phenyl sulfate | - |
? | |
additional information | may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation | Klebsiella sp. | ? | - |
? | |
additional information | may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation | Klebsiella sp. K-36 | ? | - |
? | |
phenyl sulfate + an aryl alcohol | - |
Klebsiella sp. | phenol + an aryl sulfate | - |
? | |
phenyl sulfate + an aryl alcohol | - |
Klebsiella sp. K-36 | phenol + an aryl sulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 73000, SDS-PAGE | Klebsiella sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Klebsiella sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10 min, stable below | Klebsiella sp. |
60 | - |
10 min, inactivation | Klebsiella sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | 10.5 | - |
Klebsiella sp. |