BRENDA - Enzyme Database show
show all sequences of 2.8.2.22

Purification and characterization of novel sulfotransferase obtained from Klebsiella K-36, an intestinal bacterium of rat

Kim, D.H.; Kim, H.S.; Kobashi, K.; J. Biochem. 112, 456-460 (1992)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
33% inhibition at 2 mM
Klebsiella sp.
Cd2+
almost complete inhibition at 2 mM
Klebsiella sp.
Cu2+
37% inhibition at 2 mM
Klebsiella sp.
EDTA
inactivation reversed by addition of metal ions
Klebsiella sp.
Ni2+
32% inhibition at 2 mM
Klebsiella sp.
Pb2+
35% inhibition at 2 mM
Klebsiella sp.
Zn2+
38% inhibition at 2 mM
Klebsiella sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.11
-
4-nitrophenyl sulfate
with phenol as second substrate, pH 8, 37°C
Klebsiella sp.
0.66
-
phenol
with 4-nitrophenyl sulfate as second substrate, pH 8, 37°C
Klebsiella sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
33% increase at 2 mM
Klebsiella sp.
Mg2+
65% increase at 2 mM
Klebsiella sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
73000
-
2 * 73000, SDS-PAGE
Klebsiella sp.
160000
-
gel filtration
Klebsiella sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Klebsiella sp.
may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation
?
-
-
-
additional information
Klebsiella sp. K-36
may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella sp.
-
K-36
-
Klebsiella sp. K-36
-
K-36
-
Purification (Commentary)
Commentary
Organism
-
Klebsiella sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
8.82
-
-
Klebsiella sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-acetylphenyl sulfate + phenol
28% of the activity with 4-nitrophenyl sulfate
645840
Klebsiella sp.
4-acetylphenol + phenyl sulfate
-
-
-
?
4-methylumbelliferyl sulfate + phenol
55% of the activity with p-nitrophenyl sulfate
645840
Klebsiella sp.
4-methylumbelliferol + phenyl sulfate
-
-
-
?
4-nitrophenyl sulfate + 3-chlorophenol
-
645840
Klebsiella sp.
3-chlorophenyl sulfate + 4-nitrophenol
-
-
-
?
4-nitrophenyl sulfate + 3-chlorophenol
-
645840
Klebsiella sp. K-36
3-chlorophenyl sulfate + 4-nitrophenol
-
-
-
?
4-nitrophenyl sulfate + 9-phenanthrol
-
645840
Klebsiella sp.
9-phenanthroyl sulfate + 4-nitrophenol
-
-
-
?
4-nitrophenyl sulfate + 9-phenanthrol
-
645840
Klebsiella sp. K-36
9-phenanthroyl sulfate + 4-nitrophenol
-
-
-
?
4-nitrophenyl sulfate + alpha-naphthol
-
645840
Klebsiella sp.
4-nitrophenol + 1-napththyl sulfate
-
-
-
?
4-nitrophenyl sulfate + alpha-naphthol
-
645840
Klebsiella sp. K-36
4-nitrophenol + 1-napththyl sulfate
-
-
-
?
4-nitrophenyl sulfate + phenol
-
645840
Klebsiella sp.
phenyl sulfate + 4-nitrophenol
-
-
-
?
estrone sulfate + phenol
11% of the activity with p-nitrophenyl sulfate
645840
Klebsiella sp.
estrone + phenyl sulfate
-
-
-
?
additional information
may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation
645840
Klebsiella sp.
?
-
-
-
-
additional information
may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation
645840
Klebsiella sp. K-36
?
-
-
-
-
phenyl sulfate + an aryl alcohol
-
645840
Klebsiella sp.
phenol + an aryl sulfate
-
-
-
?
phenyl sulfate + an aryl alcohol
-
645840
Klebsiella sp. K-36
phenol + an aryl sulfate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 73000, SDS-PAGE
Klebsiella sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Klebsiella sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
10 min, stable below
Klebsiella sp.
60
-
10 min, inactivation
Klebsiella sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
10.5
-
Klebsiella sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
33% inhibition at 2 mM
Klebsiella sp.
Cd2+
almost complete inhibition at 2 mM
Klebsiella sp.
Cu2+
37% inhibition at 2 mM
Klebsiella sp.
EDTA
inactivation reversed by addition of metal ions
Klebsiella sp.
Ni2+
32% inhibition at 2 mM
Klebsiella sp.
Pb2+
35% inhibition at 2 mM
Klebsiella sp.
Zn2+
38% inhibition at 2 mM
Klebsiella sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.11
-
4-nitrophenyl sulfate
with phenol as second substrate, pH 8, 37°C
Klebsiella sp.
0.66
-
phenol
with 4-nitrophenyl sulfate as second substrate, pH 8, 37°C
Klebsiella sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
33% increase at 2 mM
Klebsiella sp.
Mg2+
65% increase at 2 mM
Klebsiella sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
73000
-
2 * 73000, SDS-PAGE
Klebsiella sp.
160000
-
gel filtration
Klebsiella sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Klebsiella sp.
may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation
?
-
-
-
additional information
Klebsiella sp. K-36
may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Klebsiella sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
8.82
-
-
Klebsiella sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-acetylphenyl sulfate + phenol
28% of the activity with 4-nitrophenyl sulfate
645840
Klebsiella sp.
4-acetylphenol + phenyl sulfate
-
-
-
?
4-methylumbelliferyl sulfate + phenol
55% of the activity with p-nitrophenyl sulfate
645840
Klebsiella sp.
4-methylumbelliferol + phenyl sulfate
-
-
-
?
4-nitrophenyl sulfate + 3-chlorophenol
-
645840
Klebsiella sp.
3-chlorophenyl sulfate + 4-nitrophenol
-
-
-
?
4-nitrophenyl sulfate + 3-chlorophenol
-
645840
Klebsiella sp. K-36
3-chlorophenyl sulfate + 4-nitrophenol
-
-
-
?
4-nitrophenyl sulfate + 9-phenanthrol
-
645840
Klebsiella sp.
9-phenanthroyl sulfate + 4-nitrophenol
-
-
-
?
4-nitrophenyl sulfate + 9-phenanthrol
-
645840
Klebsiella sp. K-36
9-phenanthroyl sulfate + 4-nitrophenol
-
-
-
?
4-nitrophenyl sulfate + alpha-naphthol
-
645840
Klebsiella sp.
4-nitrophenol + 1-napththyl sulfate
-
-
-
?
4-nitrophenyl sulfate + alpha-naphthol
-
645840
Klebsiella sp. K-36
4-nitrophenol + 1-napththyl sulfate
-
-
-
?
4-nitrophenyl sulfate + phenol
-
645840
Klebsiella sp.
phenyl sulfate + 4-nitrophenol
-
-
-
?
estrone sulfate + phenol
11% of the activity with p-nitrophenyl sulfate
645840
Klebsiella sp.
estrone + phenyl sulfate
-
-
-
?
additional information
may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation
645840
Klebsiella sp.
?
-
-
-
-
additional information
may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation
645840
Klebsiella sp. K-36
?
-
-
-
-
phenyl sulfate + an aryl alcohol
-
645840
Klebsiella sp.
phenol + an aryl sulfate
-
-
-
?
phenyl sulfate + an aryl alcohol
-
645840
Klebsiella sp. K-36
phenol + an aryl sulfate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 73000, SDS-PAGE
Klebsiella sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Klebsiella sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
10 min, stable below
Klebsiella sp.
60
-
10 min, inactivation
Klebsiella sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
10.5
-
Klebsiella sp.
Other publictions for EC 2.8.2.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737843
Roubalova
Sulfation modulates the cell u ...
Desulfitobacterium hafniense
Bioorg. Med. Chem.
23
5402-5409
2015
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739142
Tang
A two-step sulfation in antibi ...
Streptomyces sp., Streptomyces sp. MK730-62F2
Nat. Chem. Biol.
9
610-615
2013
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723897
Van Der Horst
-
Sulfation of various alcoholic ...
Desulfitobacterium hafniense
Adv. Synth. Catal.
354
3501-3508
2012
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1
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4
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1
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1
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33
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1
1
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33
1
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1
-
1
1
-
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-
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725393
Kaysser
A new arylsulfate sulfotransfe ...
Streptomyces sp., Streptomyces sp. MK730-62F2
J. Biol. Chem.
285
12684-12694
2010
1
-
1
-
3
-
1
4
-
1
1
-
-
5
-
-
1
-
-
-
-
-
8
1
-
-
-
2
1
1
-
-
2
-
-
1
-
1
-
-
3
-
-
1
2
4
-
1
1
-
-
-
-
1
-
-
-
-
8
1
-
-
-
2
1
1
-
-
-
-
-
-
-
-
693650
Grimshaw
DsbL and DsbI form a specific ...
Escherichia coli
J. Mol. Biol.
380
667-680
2008
1
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1
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1
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1
1
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2
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1
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1
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1
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1
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1
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1
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2
1
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706490
Malojcic
A structural and biochemical b ...
Escherichia coli, Escherichia coli CFT073
Proc. Natl. Acad. Sci. USA
105
19217-19222
2008
-
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1
2
1
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2
2
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6
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8
1
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1
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1
2
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1
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1
2
2
1
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2
2
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8
1
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1
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672493
Kim
Cloning, expression and purifi ...
Eubacterium sp., Eubacterium sp. A-44
Biol. Pharm. Bull.
30
11-14
2007
-
-
1
-
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1
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10
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1
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8
1
1
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1
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1
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1
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1
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2
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8
1
1
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1
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660838
Kwon
Role of disulfide bond of aryl ...
Lelliottia amnigena
Arch. Pharm. Res.
28
561-565
2005
1
-
1
-
7
-
-
-
-
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1
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2
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2
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7
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2
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645849
Mozhaev
Arylsulfotransferase from Clos ...
Clostridium innocuum, Clostridium innocuum 554
Biotechnol. Bioeng.
78
567-575
2002
-
-
-
-
-
-
-
-
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2
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10
2
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1
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1
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10
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1
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4
1
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645844
Kang
Cloning, sequence analysis, an ...
Citrobacter freundii
Arch. Pharm. Res.
24
316-322
2001
-
-
1
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7
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6
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6
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645847
Kwon
Kinetic mechanism and identifi ...
Lelliottia amnigena, Lelliottia amnigena AR-37
Biochem. Biophys. Res. Commun.
285
526-529
2001
-
-
1
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11
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2
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4
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1
1
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2
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4
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1
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11
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2
4
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1
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2
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645843
Goldberg
Purification, cloning, and cha ...
Eubacterium rectale, Eubacterium rectale IIIH
J. Ind. Microbiol. Biotechnol.
25
305-309
2000
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1
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1
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645846
Kwon
Molecular cloning of the aryls ...
Lelliottia amnigena, Lelliottia amnigena AR-37
Protein Expr. Purif.
17
366-372
1999
-
-
1
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2
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2
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8
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1
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1
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14
1
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1
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1
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1
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2
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2
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1
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1
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14
1
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1
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1
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-
-
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-
645841
Bidwell
Bacterial expression of two hu ...
Homo sapiens
Chem. Biol. Interact.
109
137-141
1998
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1
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1
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1
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1
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1
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645845
Konishi-Imamura
Regulation of arylsulfate sulf ...
Eubacterium sp.
J. Enzyme Inhib.
8
233-241
1995
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6
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-
2
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3
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2
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2
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4
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6
4
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2
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2
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2
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645848
Lee
Purification and reaction mech ...
Haemophilus sp., Haemophilus sp. K-12
J. Biochem.
118
796-801
1995
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-
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7
2
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2
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5
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1
1
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3
1
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11
1
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4
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7
4
2
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2
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1
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3
1
-
11
1
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645839
Konishi-Imamura
Improved purification of aryls ...
Eubacterium sp., Eubacterium sp. A-44
J. Biochem.
115
1097-1100
1994
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2
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11
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1
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2
1
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4
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2
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1
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2
1
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4
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-
645842
Muramatsu
Enzymic O-sulfation of tyrosin ...
Eubacterium sp., Eubacterium sp. A-44
Eur. J. Biochem.
223
243-248
1994
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7
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2
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2
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645840
Kim
Purification and characterizat ...
Klebsiella sp., Klebsiella sp. K-36
J. Biochem.
112
456-460
1992
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7
2
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2
2
2
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2
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1
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1
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14
1
1
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2
-
1
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7
-
2
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2
2
2
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1
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-
1
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14
1
1
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2
-
1
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645837
Kobashi
-
A novel type of arylsulfotrans ...
Eubacterium sp., Eubacterium sp. A-44
J. Protein Chem.
6
237-244
1987
2
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1
2
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7
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1
1
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14
1
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1
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2
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1
2
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1
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14
1
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1
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645838
Kim
Purification, characterization ...
Eubacterium sp., Eubacterium sp. A-44
Biochim. Biophys. Acta
872
33-41
1986
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1
11
2
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2
2
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-
7
-
-
1
1
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-
1
1
8
1
1
-
2
-
3
-
1
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-
1
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-
1
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11
-
2
-
2
2
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1
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1
1
8
1
1
-
2
-
3
-
1
1
-
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-
645850
Kobashi
A novel sulfotransferase sulfa ...
Eubacterium sp., Eubacterium sp. A-44
Biochem. Biophys. Res. Commun.
140
38-42
1986
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7
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1
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12
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1
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1
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12
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1
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