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Literature summary for 2.8.2.20 extracted from
- Preparation of tyrosylprotein sulfotransferases for in vitro one-pot enzymatic synthesis of sulfated proteins/peptides (2018), ACS Omega, 3, 11633-11642 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | usage of tyrosylprotein sulfotransferases for in vitro one-pot enzymatic synthesis of sulfated proteins/peptides | Homo sapiens |
| synthesis | usage of tyrosylprotein sulfotransferases for in vitro one-pot enzymatic synthesis of sulfated proteins/peptides | Drosophila melanogaster |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Tpst, recombinant expression of His-tagged enzyme fused to GST or NusA in Escherichia coli strain BL21(DE3), method optimization, overview | Drosophila melanogaster |
| gene TPST1, recombinant expression of His-tagged enzyme fused to GST or NusA in Escherichia coli strain BL21(DE3), method optimization, overview | Homo sapiens |
| gene TPST2, recombinant expression of His-tagged enzyme fused to GST or NusA in Escherichia coli strain BL21(DE3), method optimization, overview | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi membrane | - |
Homo sapiens | 139 | - |
| Golgi membrane | - |
Drosophila melanogaster | 139 | - |
| membrane | a type II membrane enzyme | Homo sapiens | 16020 | - |
| membrane | a type II membrane enzyme | Drosophila melanogaster | 16020 | - |
| trans-Golgi network | - |
Homo sapiens | 5802 | - |
| trans-Golgi network | - |
Drosophila melanogaster | 5802 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + protein tyrosine | Homo sapiens | - |
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate | - |
? |  |
| 3'-phosphoadenylyl sulfate + protein tyrosine | Drosophila melanogaster | - |
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | Q9VYB7 | - |
- |
| Homo sapiens | O60507 | - |
- |
| Homo sapiens | O60704 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| preparation of TPST from Golgi-enriched membrane fractions, recombinant His-tagged GST- or NusA-fused enzyme from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, low solubility or contamination with tag material | Homo sapiens |
| preparation of TPST from Golgi-enriched membrane fractions, recombinant His-tagged GST- or NusA-fused enzyme from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, low solubility or contamination with tag material | Drosophila melanogaster |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1 | recombinantly expressed substrate PSGL-1, glycoprotein found on the plasma membrane of neutrophils or monocytes. TPST activity on GST-tagged PSGL-1 is not affected by the GST fusion tag | Homo sapiens | ? | - |
? | |
| 3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1 | recombinantly expressed substrate PSGL-1, glycoprotein found on the plasma membrane of neutrophils or monocytes. TPST activity on GST-tagged PSGL-1 is not affected by the GST fusion tag | Drosophila melanogaster | ? | - |
? | |
| 3'-phosphoadenylyl sulfate + protein tyrosine | - |
Homo sapiens | adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + protein tyrosine | - |
Drosophila melanogaster | adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DmTPST | - |
Homo sapiens |
| DmTPST | - |
Drosophila melanogaster |
| TPST | - |
Homo sapiens |
| TPST | - |
Drosophila melanogaster |
| TPST-1 | - |
Homo sapiens |
| TPST-2 | - |
Homo sapiens |
| TPST1 | - |
Homo sapiens |
| TPST2 | - |
Homo sapiens |
| tyrosylprotein sulfotransferase | - |
Homo sapiens |
| tyrosylprotein sulfotransferase | - |
Drosophila melanogaster |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | protein tyrosine sulfation (PTS), catalyzed by membrane-anchored tyrosylprotein sulfotransferase (TPST), is one of the most common posttranslational modifications of secretory and transmembrane proteins. PTS, a key modulation of extracellular protein-protein interactions, accounts for various important biological activities, namely, virus entry, inflammation, coagulation, and sterility. PTS occurs in the trans-Golgi network, and the sulfated proteins and peptides are typically transmembrane or secretory proteins | Homo sapiens |
| physiological function | protein tyrosine sulfation (PTS), catalyzed by membrane-anchored tyrosylprotein sulfotransferase (TPST), is one of the most common posttranslational modifications of secretory and transmembrane proteins. PTS, a key modulation of extracellular protein-protein interactions, accounts for various important biological activities, namely, virus entry, inflammation, coagulation, and sterility. PTS occurs in the trans-Golgi network, and the sulfated proteins and peptides are typically transmembrane or secretory proteins | Drosophila melanogaster |
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