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Literature summary for 2.8.2.20 extracted from

  • Hoffhines, A.J.; Damoc, E.; Bridges, K.G.; Leary, J.A.; Moore, K.L.
    Detection and purification of tyrosine-sulfated proteins using a novel anti-sulfotyrosine monoclonal antibody (2006), J. Biol. Chem., 281, 37877-37887.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.20

Protein Variants

Protein Variants Comment Organism
additional information generation of Tpst1 and Tpst2 double knock-out mice Mus musculus
additional information generation of Tpst1 and Tpst2 double knock-out mice, certain sperm/epididymal proteins are undersulfated in Tpst2-/- mice Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Mus musculus 5794
-
membrane TPST1 is a transmembrane enzyme Mus musculus 16020
-
membrane TPST2 are transmembrane enzymes Mus musculus 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus protein tyrosine O-sulfation is a post-translational modification mediated by the Golgi tyrosylprotein sulfotransferase, TPST1, that catalyzes the transfer of sulfate to tyrosine residues in secreted and transmembrane proteins, tyrosine sulfation plays a role in protein-protein interactions, overview ?
-
 ?
additional information Mus musculus protein tyrosine O-sulfation is a post-translational modification mediated by the Golgi tyrosylprotein sulfotransferase, TPST2, that catalyzes the transfer of sulfate to tyrosine residues in secreted and transmembrane proteins, tyrosine sulfation plays a role in protein-protein interactions, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 isozyme TPST1
-
Mus musculus
-
 isozyme TPST2
-

Purification (Commentary)

Purification (Comment) Organism
develoment of an immunoaffinity purification method using anti-sulfotyrosine monoclonal antibody PSG2, recombinantly expressed in CHO cells Mus musculus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 development of a specific detection method using an anti-sulfotyrosine monoclonal antibody, termed PSG2, that binds with high affinity and exquisite specificity to sulfotyrosine residues in peptides and proteins independently of sequence context, overview Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information protein tyrosine O-sulfation is a post-translational modification mediated by the Golgi tyrosylprotein sulfotransferase, TPST1, that catalyzes the transfer of sulfate to tyrosine residues in secreted and transmembrane proteins, tyrosine sulfation plays a role in protein-protein interactions, overview Mus musculus ?
-
 ?
additional information protein tyrosine O-sulfation is a post-translational modification mediated by the Golgi tyrosylprotein sulfotransferase, TPST2, that catalyzes the transfer of sulfate to tyrosine residues in secreted and transmembrane proteins, tyrosine sulfation plays a role in protein-protein interactions, overview Mus musculus ?
-
 ?
additional information isozyme Tpst1 shows differences in the macromolecular substrate specificities, overview Mus musculus ?
-
 ?
additional information isozyme Tpst2 shows differences in the macromolecular substrate specificities, overview Mus musculus ?
-
 ?

Subunits

Subunits Comment Organism
More epitope mapping Mus musculus

Synonyms

Synonyms Comment Organism
TPST1
-
 Mus musculus
TPST2
-
 Mus musculus