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Literature summary for 2.8.2.20 extracted from

  • Mishiro, E.; Sakakibara, Y.; Liu, M.; Suiko, M.
    Differential enzymatic characteristics and tissue-specific expression of human TPST-1 and TPST-2 (2006), J. Biochem., 140, 731-737.
    View publication on PubMed
Search for more literature for 2.8.2.20

Cloned(Commentary)

Cloned (Comment) Organism
expression of isozyme TPST-1 in HEK-293T cells, quantitative real-time PCR expression analysis Homo sapiens
expression of isozyme TPST-2 in HEK-293T cells, quantitative real-time PCR expression analysis Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Homo sapiens
0.0097
-
 PSGL-1 pH 6.0, 30°C, isozyme TPST-1 Homo sapiens
0.0175
-
 Tyr-Tyr-Tyr pH 6.0, 30°C, isozyme TPST-1 Homo sapiens
0.022
-
 Tyr-Tyr-Tyr pH 6.0, 30°C, isozyme TPST-2 Homo sapiens
0.0269
-
 PSGL-1 pH 6.0, 30°C, isozyme TPST-2 Homo sapiens
1.223
-
 benzyl-Glu-Tyr pH 6.0, 30°C, isozyme TPST-2 Homo sapiens
1.297
-
 benzyl-Glu-Tyr pH 6.0, 30°C, isozyme TPST-1 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol TPST-1 displays type II transmembrane topology with a short N-terminal cytoplasmic domain, a single 17-residue transmembrane domain, and a luminal catalytic domain Homo sapiens 5829
-
cytosol TPST-2 displays type II transmembrane topology with a short N-terminal cytoplasmic domain, a single 17-residue transmembrane domain, and a luminal catalytic domain Homo sapiens 5829
-
membrane TPST-1 displays type II transmembrane topology with a short N-terminal cytoplasmic domain, a single 17-residue transmembrane domain, and a luminal catalytic domain Homo sapiens 16020
-
membrane TPST-2 displays type II transmembrane topology with a short N-terminal cytoplasmic domain, a single 17-residue transmembrane domain, and a luminal catalytic domain Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates isozyme TPST-2 Homo sapiens
Mn2+ activates isozymes TPST-1 Homo sapiens
additional information no activation of isozyme TPST-1 by Mg2+ Homo sapiens
additional information no activation of isozyme TPST-2 by Mn2+ Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl sulfate + protein tyrosine Homo sapiens
-
 adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O60507 TPST-1; isozyme TPST-1
-
Homo sapiens O60704 TPST-2; isozyme TPST-2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein TPST-1 has two putative N-glycosylation sites Homo sapiens
glycoprotein TPST-2 has two putative N-glycosylation sites Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain adult and fetal Homo sapiens
-
heart
-
 Homo sapiens
-
liver
-
 Homo sapiens
-
additional information broad tissue distribution of isozyme TPST-1, overview Homo sapiens
-
additional information broad tissue distribution of isozyme TPST-2, overview Homo sapiens
-
neuron
-
 Homo sapiens
-
placenta
-
 Homo sapiens
-
prostate gland
-
 Homo sapiens
-
salivary gland
-
 Homo sapiens
-
spinal cord
-
 Homo sapiens
-
testis
-
 Homo sapiens
-
thymus
-
 Homo sapiens
-
uterus
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000031
-
 recombinant TPST-1 in HEK-293T cell extract Homo sapiens
0.0000032
-
 recombinant TPST-2 in HEK-293T cell extract Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + benzyl-Glu-Tyr
-
 Homo sapiens adenosine 3',5'-bisphosphate + benzyl-Glu-Tyr-O-sulfate
-
 ?
3'-phosphoadenylyl sulfate + protein tyrosine
-
 Homo sapiens adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
 ?
3'-phosphoadenylyl sulfate + protein tyrosine high activity, isozyme TPST-2 Homo sapiens adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
 ?
3'-phosphoadenylyl sulfate + protein tyrosine low activity, isozyme TPST-1 Homo sapiens adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
 ?
3'-phosphoadenylyl sulfate + PSGL-1
-
 Homo sapiens adenosine 3',5'-bisphosphate + PSGL-1 sulfate
-
 ?
3'-phosphoadenylyl sulfate + Tyr-Tyr-Tyr
-
 Homo sapiens adenosine 3',5'-bisphosphate + Tyr-Tyr-Tyr-O-sulfate
-
 ?
additional information activity with peptides encompassing tyrosine sulfation sites of chemokine receptors, CCR2, CCR3, CCR5, CCR8, cholecystokinin CCK, and complement C4, overview Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
More TPST-1 displays type II transmembrane topology with a short N-terminal cytoplasmic domain, a single 17-residue transmembrane domain, and a luminal catalytic domain Homo sapiens
More TST-2 displays type II transmembrane topology with a short N-terminal cytoplasmic domain, a single 17-residue transmembrane domain, and a luminal catalytic domain Homo sapiens

Synonyms

Synonyms Comment Organism
TPST-1
-
 Homo sapiens
TPST-2
-
 Homo sapiens
tyrosylprotein sulfotransferase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 TPST-2 Homo sapiens
6.5
-
 TPST-1 Homo sapiens