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Literature summary for 2.8.2.20 extracted from
- Zebrafish tyrosylprotein sulfotransferase: molecular cloning, expression, and functional characterization (2004), Biochem. Cell Biol., 82, 295-303.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in COS-7 cells | Danio rerio |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Danio rerio |  |
| Cd2+ | - |
Danio rerio | |
| Cu2+ | 10 mM, complete inhibition | Danio rerio | |
| Fe2+ | 10 mM, complete inhibition | Danio rerio | |
| Hg2+ | 10 mM, complete inhibition | Danio rerio | |
| Pb2+ | - |
Danio rerio | |
| Zn2+ | 10 mM, complete inhibition | Danio rerio | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0052 | - |
ATEYEFLDYDFL | pH 5.8, 28°C | Danio rerio | |
| 0.0085 | - |
ATEFEYLDYDFL | pH 5.8, 28°C | Danio rerio | |
| 0.0315 | - |
ATEFEFLDYDFL | pH 5.8, 28°C | Danio rerio | |
| 0.0359 | - |
ATEYEYLDYDFL | pH 5.8, 28°C | Danio rerio | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | type II transmembrane protein | Danio rerio | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 10 mM, stimulates | Danio rerio | |
| Mn2+ | 20 mM, 2.5fold stimulation | Danio rerio | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Danio rerio | Q6WZB4 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl-sulfate + ATEFEFLDYDFL | - |
Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylyl-sulfate + ATEFEYLDYDFL | - |
Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylyl-sulfate + ATEYEFLDYDFL | - |
Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylyl-sulfate + ATEYEYLDYDFL | - |
Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TPST | - |
Danio rerio |
| tyrosylprotein sulfotransferase | - |
Danio rerio |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | - |
- |
Danio rerio |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6.5 | pH 5.5: about 95% of maximal activity, pH 6.5: about 55% of maximal activity | Danio rerio |
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