General Stability | Organism |
---|---|
stable in cell lysates prepared by freeze-thawing in 100 mM HEPES-NaOH buffer, pH 7.4, 20 mM MgCl2, 10 mM mercaptoethanol or in 5 mM EDTA or 0.32 M sucrose or by hypoosmotic lysis using 5 mM EDTA, no activity in cell lysates prepared in the presence of Triton X-100 or by sonication | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 5 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0083 | - |
3'-phosphoadenylylsulfate | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Rattus norvegicus | 16020 | - |
additional information | subcellular distribution | Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
divalent cations | requirement | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + protein tyrosine | Rattus norvegicus | involved in post-translational processing of specific PC12 cell proteins | adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
PC-12 cell | fast-responding clone | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + acidic polypeptide tyrosine | polypeptides from PC12 cells, referred to as p113, p105, p86 and p84, according to their average MW | Rattus norvegicus | adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate | - |
? | |
3'-phosphoadenylylsulfate + acidic polypeptide tyrosine | specifically sulfates Tyr-residues adjacent to acidic amino acids | Rattus norvegicus | adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate | - |
? | |
3'-phosphoadenylylsulfate + protein tyrosine | involved in post-translational processing of specific PC12 cell proteins | Rattus norvegicus | adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |