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Literature summary for 2.8.2.2 extracted from
- Upregulation of hydroxysteroid sulfotransferase 2B1b promotes hepatic oval cell proliferation by modulating oxysterol-induced LXR activation in a mouse model of liver injury (2017), Arch. Toxicol., 91, 271-287 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Sult2B1, real-time quantitative PCR enzyme expression analysis | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the male and female homozygous SULT2B1 knockout mice, SULT2B1 knockout mouse line, B6;129-Sult2b1tm1Rus/J, are used in which exons 4-8 of the SULT2B1 gene are removed abolishing the gene expression, but the mutant mice are viable and fertile. They lack cholesterol sulfate in the dermis but otherwise appear to have normal lipid metabolism. The mouse liver injury model is established utilizing the SULT2B1-/- mice and their wild-type littermates. To induce liver injury, male C57BL/6 mice (age, 6-8 weeks) are fed a diet containing 0.1% DDC or 0.025% alpha-naphthylisothiocyanate (ANIT) for 4 weeks, or intraperitoneally injected with carbon tetrachloride (0.4 ml/kg body weight) or thioacetamide (200 mg/kg) twice a week for 4 weeks. SULT2B1-deficient HOCs display decreased proliferative capability, and IL-6/STAT3 signaling is involved in the effect of SULT2B1b on HOC proliferation. Oxysterol-induced LXR activation is enhanced in SULT2B1-deficient liver | Mus musculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mus musculus | 5829 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + cholesterol | Mus musculus | - |
adenosine 3',5'-bisphosphate + cholesterol 3-sulfate | - |
? |  |
| 3'-phosphoadenylyl sulfate + cholesterol | Mus musculus C57BL/6 | - |
adenosine 3',5'-bisphosphate + cholesterol 3-sulfate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | O35400 | - |
- |
| Mus musculus C57BL/6 | O35400 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatic oval cell | - |
Mus musculus | - |
| hepatocyte | - |
Mus musculus | - |
| hepatoma cell | SULT2B1b mRNA levels in clinical hepatocarcinoma tumor samples are higher than in the non-tumorous tissue adjacent to the tumors | Mus musculus | - |
| liver | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + cholesterol | - |
Mus musculus | adenosine 3',5'-bisphosphate + cholesterol 3-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + cholesterol | - |
Mus musculus C57BL/6 | adenosine 3',5'-bisphosphate + cholesterol 3-sulfate | - |
? | |
| additional information | hydroxysteroid sulfotransferase 2B1b (SULT2B1b) is highly selective for the sulfation of 3beta-hydroxysteroids such as cholesterol, oxysterols, DHEA, D5-adiol, 5alpha-androstane-3beta, 17beta-diol (anstane-diol), and pregnenolone | Mus musculus | ? | - |
- |
|
| additional information | hydroxysteroid sulfotransferase 2B1b (SULT2B1b) is highly selective for the sulfation of 3beta-hydroxysteroids such as cholesterol, oxysterols, DHEA, D5-adiol, 5alpha-androstane-3beta, 17beta-diol (anstane-diol), and pregnenolone | Mus musculus C57BL/6 | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hydroxysteroid sulfotransferase 2B1b | - |
Mus musculus |
| SULT2B1 | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Mus musculus |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Mus musculus | the expression of SULT2B1b is increased dramatically in a chemical-induced liver injury model (using a hepatotoxic diet containing 0.1% 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC)), mainly in hepatic oval cells (HOCs) | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | upregulation of hydroxysteroid sulfotransferase 2B1b promotes hepatic oval cell (HOC) proliferation by modulating oxysterol-induced LXR activation in a mouse model of liver injury. HOCs derived from SULT2B1-/- mice show lower proliferative capability than those from wild-type mice. SULT2B1b overexpression promotes growth of the WB-F344 hepatic oval cell line, whereas SULT2B1b knockdown inhibits growth of these cells. The levels of 22-hydroxycholesterol, 25-hydroxycholesterol, and 24,25-epoxycholesterol(all physiological ligands of liver X receptors) are higher in the DDC-injured livers of SULT2B1-/- mice than in livers of wild-type mice. Knockdown of SULT2B1b expression inhibited proliferation of mouse and human hepatocellular carcinoma cells (Hepa1-6, Huh-7, SMMC-7721, and BEL-7402), whereas overexpression of SULT2B1b promoted such proliferation in vitro and in vivo | Mus musculus |
| physiological function | hydroxysteroid sulfotransferase 2B1b (SULT2B1b) sulfates cholesterol and oxysterols. Hepatic oval cells (HOCs), thought to be progenitor cells, can be triggered in chemically injured livers. Role of SULT2B1b in HOC proliferation after liver injury, overview. The IL-6/STAT3 signaling pathway is promoted by SULT2B1b. Liver X receptors (LXRs) activation inhibits HOC proliferation and the IL-6/STAT3 signaling pathway, and these effects can be reversed by SULT2B1b activity. SULT2B1b promotes IL-6/STAT3 signaling and HOC proliferation by suppressing oxysterol-induced LXR activation | Mus musculus |
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