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Literature summary for 2.8.2.2 extracted from
- Detoxification function of the Arabidopsis sulphotransferase AtSOT12 by sulphonation of xenobiotics (2015), Plant Cell Environ., 38, 1673-1682.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Saccharomyces cerevisiae strain w303-1a | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
cycloheximide | at pH 7.5 and 30°C | Arabidopsis thaliana |  |
| 0.035 | - |
o-Cresol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.135 | - |
4-Ethylphenol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.152 | - |
3-Ethylphenol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.18 | - |
m-cresol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.276 | - |
4-nitrophenol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.312 | - |
2-Nitrophenol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.475 | - |
3-Nitrophenol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.494 | - |
p-Cresol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 1.16 | - |
2-Ethylphenol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Arabidopsis thaliana | 5829 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | P52839 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Arabidopsis thaliana | - |
| seedling | - |
Arabidopsis thaliana | - |
| shoot | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + 1,10-phenanthroline | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylylsulfate + 1-hydroxypyrene | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + 1-hydroxypyrene sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + 1-naphthol | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + 1-naphthyl sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + 1-pyrenemethanol | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + 1-pyrenemethylsulfate | - |
? | |
| 3'-phosphoadenylylsulfate + 2-aminofluorene | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + 2-aminofluorene sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + 2-aminophenol | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + 2-aminophenyl sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + 2-ethylphenol | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + 2-ethylphenyl sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + 2-hydroxyestradiol | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + 2-hydroxyestradiol sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + 2-naphthol | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + 2-naphthyl sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + 2-nitrophenol | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + 2-nitrophenyl sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + 3-aminophenol | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + 3-aminophenyl sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + 3-ethylphenol | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + 3-ethylphenyl sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + 3-nitrophenol | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + 3-nitrophenyl sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + 4-aminophenol | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + 4-aminophenyl sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + 4-ethylphenol | best substrate | Arabidopsis thaliana | adenosine 3',5'-bisphosphate + 4-ethylphenyl sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + 4-nitrophenol | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + 5-sulfosalicylic acid | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + 5-sulfo-2-(sulfooxy)benzoic acid | - |
? | |
| 3'-phosphoadenylylsulfate + beta-estradiol | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + beta-estradiol 3-O-sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + cycloheximide | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + cycloheximide sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + hycanthone | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + hycanthone sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + hydroquinone | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + hydroquinone sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + m-cresol | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + m-cresol sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + o-cresol | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + o-cresol sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + p-cresol | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + p-cresol sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + sodium 4-amino-salicylate | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + sodium 4-amino-salicylate sulfate | - |
? | |
| additional information | no activity with pentachlorophenol | Arabidopsis thaliana | ? | - |
? | |
| additional information | the enzyme displays relatively low activities to steroid compounds, and although the enzyme sulfonates a variety of phenolic compounds, it has selectivity towards side chains and their locations at the phenolic ring | Arabidopsis thaliana | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SOT12 | - |
Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000035 | - |
o-Cresol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.00004 | - |
m-cresol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.000053 | - |
3-Ethylphenol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.000109 | - |
4-Ethylphenol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.000112 | - |
2-Ethylphenol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.000128 | - |
p-Cresol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.000389 | - |
3-Nitrophenol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.000459 | - |
2-Nitrophenol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.000545 | - |
4-nitrophenol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | isoform SOT12 is required for detoxification of cycloheximide | Arabidopsis thaliana |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000097 | - |
2-Ethylphenol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.000222 | - |
m-cresol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.000259 | - |
p-Cresol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.000347 | - |
3-Ethylphenol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.000812 | - |
4-Ethylphenol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.000819 | - |
3-Nitrophenol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.000849 | - |
o-Cresol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.00147 | - |
2-Nitrophenol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.001975 | - |
4-nitrophenol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
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