Cloned (Comment) | Organism |
---|---|
expression of GFP-tagged wild-type allozymes and mutant truncated SULT2B1a and SULT2B1b variants lacking the SULT2B1 proline-rich tail in COS-1 cells | Homo sapiens |
isozymes SULT2B1a and SULT2B1b are encoded by a single SULT2B1 gene as a result of alternative transcription initiation and alternative splicing, genotyping of African-American and Caucasian-American individuals, transient expression of GFP-tagged SULT2B1a and SULT2B1b in COS-1 cells, in vitro transcription and translation of truncated SULT2B1a and SULT2B1b variants that lack the SULT2B1 proline-rich tail using rabbit reticulocyte lysate | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D176N | naturally occuring polymorphism in SULT2B1a | Homo sapiens |
L36S | naturally occuring polymorphism in SULT2B1a | Homo sapiens |
additional information | construction of truncated SULT2B1a and SULT2B1b variants that lack the SULT2B1 proline-rich tail, genotype-to-phenotype approach to identify and characterize common sequence variation in SULT2B1, determination and analysis of 100 polymorphisms in African-American and Caucasian-American individuals, including four nonsynonymous coding single nucleotide polymorphisms and one 6-base pair deletion, within the shared region of the open reading frame, overview | Homo sapiens |
additional information | construction of truncated SULT2b1a and SULT2B1b variants by site-directed mutagenesis to introduce a translation termination codon immediately before the final 53 amino acids at the C terminus of the protein. Pharmacogenomics, gene sequence variation and functional genomics, determination of 100 polymorphisms, including four nonsynonymous coding single nucleotide polymorphisms and one 6-base pair deletion, in African-American and Caucasian-American individuals, overview | Homo sapiens |
P330L | naturally occuring polymorphism in SULT2B1a | Homo sapiens |
R215H | naturally occuring polymorphism in SULT2B1a | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
additional information | subcellular localization of isozymes SULT2B1a and SULT2B1b, overview | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozymes SULT2B1a and SULT2B1b | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
MCF-7 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + cholesterol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + cholesterol 3-sulfate | - |
? | |
3'-phosphoadenylyl sulfate + cholesterol | preferred substrate of SULT2B1b | Homo sapiens | adenosine 3',5'-bisphosphate + cholesterol 3-sulfate | - |
? | |
3'-phosphoadenylyl sulfate + dehydroepiandrosterone | - |
Homo sapiens | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
? | |
3'-phosphoadenylyl sulfate + dehydroepiandrosterone | preferred substrate of SULT2B1b | Homo sapiens | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
? | |
3'-phosphoadenylyl sulfate + pregnenolone | - |
Homo sapiens | adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate | - |
? | |
3'-phosphoadenylyl sulfate + pregnenolone | preferred substrate of SULT2B1a | Homo sapiens | adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate | - |
? | |
additional information | SULT2B1b catalyzes the sulfonation of 3beta-hydroxysteroid hormones and cholesterol, whereas SULT2B1a preferentially catalyzes pregnenolone sulfonation | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hydroxysteroid sulfotransferase | - |
Homo sapiens |
More | SULT2B1 is a member of the cytosolic SULT gene family | Homo sapiens |
SULT2B1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Homo sapiens |
5.5 | 6.5 | assay at | Homo sapiens |