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Literature summary for 2.8.2.2 extracted from
- Characterization of proline-serine-rich carboxyl terminus in human sulfotransferase 2B1b: immunogenicity, subcellular localization, kinetic properties, and phosphorylation (2006), Drug Metab. Dispos., 34, 1749-1755.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged full-length SULT2B1b and of truncated SULT2B1b lacking proline- and serine-rich carboxyl extension in Escherichia coli strain DH5alpha, expression and serine phosphorylation of SULT2B1b in transfected BeWo cells, overview | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a truncated mutant SULT2B1b lacking the 53 amino acid-proline- and serine-rich carboxyl extension, the mutant enzyme shows decreased Km for dehydroepiandrosterone and a kcat reduced by 91% compared to the wild-type full-length SULT2B1b | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the proline- and serine-rich carboxyl extension of SULT2B1b is an important site in the kinetic activity of this SULT isoform, overview | Homo sapiens | |
| 0.0006 | - |
dehydroepiandrosterone | pH 7.5, 37°C, recombinant truncated mutant SULT2B1 | Homo sapiens |  |
| 0.0038 | - |
dehydroepiandrosterone | pH 7.5, 37°C, recombinant wild-type, full-length SULT2B1 | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | the proline- and serine-rich carboxyl extension of SULT2B1b is an important site in the nuclear translocation of this SULT isoform, overview | Homo sapiens | 5829 | - |
| microsome | - |
Homo sapiens | - |
- |
| nucleus | the proline- and serine-rich carboxyl extension of SULT2B1b is an important site in the nuclear translocation of this SULT isoform, overview | Homo sapiens | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + dehydroepiandrosterone | Homo sapiens | - |
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O00204 | isozymes SULT2B1a and SULT2B1b | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | recombinant wild-type, full-length SULT2B1b, but not truncated SULT2B1b, is phosphorylated by casein kinase or Cdc2 protein kinase in vitro | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged full-length SULT2B1b and of truncated SULT2B1b lacking proline- and serine-rich carboxyl extension from Escherichia coli strain DH5alpha by nickel affinity chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + dehydroepiandrosterone | - |
Homo sapiens | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
? | |
| additional information | SULT2B1b is selective for the sulfation of 3beta-hydroxysteroids, such as DHEA, pregnenolone, androstenediol, and 5alpha-androstane-3beta,17beta-diol, no sulfation is detectable with 3alpha-hydroxysteroids or estrogens as substrates | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | SULT2B1b has an extension at the proline- and serine-rich carboxyl end of about 53 amino acids, structure and function, the proline- and serine-rich carboxyl extension of SULT2B1b is an important site in the immunogenicity, nuclear translocation, kinetic activity, and thermostability of this SULT isoform, overview | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme belongs to the SULT2 family | Homo sapiens |
| sulfotransferase 2B1b | - |
Homo sapiens |
| SULT2B1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the proline- and serine-rich carboxyl extension of SULT2B1b is an important site in the thermostability of this SULT isoform, overview | Homo sapiens |
| 42 | - |
30 min, the activity of the recombinant wild-type, full-length SULT2B1b is reduced by 30%, but the activity of the recombinant truncated SULT2B1bmutant is reduced 3.5fold more | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
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