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Literature summary for 2.8.2.2 extracted from

  • Chen, W.T.; Liu, M.C.; Yang, Y.S.
    Fluorometric assay for alcohol sulfotransferase (2005), Anal. Biochem., 339, 54-60.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-
liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
continuous fluorimetric assay for alcohol sulfotransferase whose sensitivity is comparable to that of the end-point radioactive assay. Assay procedure can be adapted for high-throughput assay using a microplate reader Escherichia coli
additional information
-
continuous fluorimetric assay for alcohol sulfotransferase whose sensitivity is comparable to that of the end-point radioactive assay. Assay procedure can be adapted for high-throughput assay using a microplate reader Sus scrofa
additional information
-
continuous fluorimetric assay for alcohol sulfotransferase whoses sensitivity is comparable to that of the end-point radioactive assay. Assay procedure can be adapted for high-throughput assay using a microplate reader Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
Rattus norvegicus adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
Sus scrofa adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
Escherichia coli adenosine 3',5'-bisphosphate + androst-5-en-17-one 3-sulfate
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
Escherichia coli BL-21 adenosine 3',5'-bisphosphate + androst-5-en-17-one 3-sulfate
-
?

Synonyms

Synonyms Comment Organism
alcohol sulfotransferase
-
Escherichia coli
alcohol sulfotransferase
-
Rattus norvegicus
alcohol sulfotransferase
-
Sus scrofa
AST
-
Escherichia coli
AST
-
Rattus norvegicus
AST
-
Sus scrofa