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Literature summary for 2.8.2.2 extracted from
- Human dehydroepiandrosterone sulfotransferase: purification and characterization of a recombinant protein (2001), J. Steroid Biochem. Mol. Biol., 77, 159-165.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DHEA-ST cloned and expressed in glutathione sulfotransferase fusion form in Escherichia coli | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0019 | - |
dehydroepiandrosterone | pH 7.5, 37°C | Homo sapiens |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
2 * 34000, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + dehydroepiandrosterone | Homo sapiens | - |
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
human | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.15 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + dehydroepiandrosterone | - |
Homo sapiens | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 34000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 45 | - |
Homo sapiens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | enzyme activity at 25°C and 30°C drops to 35% and 56%, repective to the maximum activity at 45°C, residual activity is determined to be 62% of the maximum at 50°C | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
90% basal activity remains after 15 min | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Homo sapiens |
| 7 | 9 | recombinant enzyme, expressed in Escherichia coli | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | enzyme activity at pH 5.0 is 6% of the maximum in the optimum range, 50% of activity maximum at pH 6.0, no activity is detected at either pH 4.0 or 10.0 | Homo sapiens |
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