Any feedback?
Literature summary for 2.8.2.2 extracted from
- Human dehydroepiandrosterone sulfotransferase. Purification, molecular cloning, and characterization (1995), Ann. N. Y. Acad. Sci., 774, 59-72.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DHEA-ST cloned and expressed in Salmonella typhimurium, Escherichia coli and COS cells | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33770 | - |
calculated from putative amino acid sequence, estimated from cDNA | Homo sapiens |
| 35000 | - |
2 * 35000, SDS-PAGE | Homo sapiens |
| 68000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + dehydroepiandrosterone | Homo sapiens | critical step in the provision of substrates for estrogen biosynthesis by the placenta during pregnancy, sulfation represents a major enzymatic reaction in the metabolism, excretion and homeostasis of steroid hormones and bile acids in addition to numerous other endogenous compounds and xenobiotics, regulating steroid hormone bioactivity | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
human | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | - |
Homo sapiens | - |
| epithelium | - |
Homo sapiens | - |
| fetus | - |
Homo sapiens | - |
| hepatocyte | - |
Homo sapiens | - |
| intestine | fetal intestinal villi | Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| testis | interstitial cells of the testis | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + 1'-hydroxysafrole | isoenzyme DHEA-ST, sulfation and bioactivation to a lesser extent | Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylylsulfate + 1-hydroxymethylpyrene | isoenzyme DHEA-ST, sulfation and bioactivation | Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylylsulfate + 17-beta-estradiol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + 17-beta-estradiol 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + 17alpha-ethinylestradiol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + 17alpha-ethinylestradiol sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + 6-hydroxymethylbenzo[a]pyrene | isoenzyme DHEA-ST, sulfation and bioactivation | Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylylsulfate + alpha-hydroxytamoxifen | isoenzyme DHEA-ST, sulfation and bioactivation to a lesser extent | Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylylsulfate + androstenediol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + androstenediol sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + androsterone | - |
Homo sapiens | adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + beta-estradiol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + beta-estradiol 3-O-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + cholesterol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + cholesterol sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + dehydroepiandrosterone | - |
Homo sapiens | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + dehydroepiandrosterone | critical step in the provision of substrates for estrogen biosynthesis by the placenta during pregnancy, sulfation represents a major enzymatic reaction in the metabolism, excretion and homeostasis of steroid hormones and bile acids in addition to numerous other endogenous compounds and xenobiotics, regulating steroid hormone bioactivity | Homo sapiens | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + estrone | - |
Homo sapiens | adenosine 3',5'-bisphosphate + estrone sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + hycanthone | isoenzyme DHEA-ST, sulfation and bioactivation | Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylylsulfate + N-hydroxy-2-acetylaminofluorene | isoenzyme DHEA-ST, sulfation and bioactivation to a lesser extent | Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylylsulfate + testosterone | 17-hydroxyl of testosterone | Homo sapiens | adenosine 3',5'-bisphosphate + testosterone 3-sulfate | - |
r | |
| additional information | cortisol, corticosterone, betamathasone and dexamethasone are no substrates | Homo sapiens | ? | - |
? | |
| additional information | isoform DHEA-ST, sulfate conjugation involved in bioactivation of compounds to reactive electrophilic forms, the hydroxymethyl group is sulfated and undergoes subsequent sponaneous rearrangement to release the sulfate moiety and generate an electrophilic benzylic carbocation that can react with cellular nucleophiles such as DNA, RNA, and protein | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 35000, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DHEA ST | - |
Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
