KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
3'-phosphoadenylylsulfate | pH 5.5, 37°C | Rattus norvegicus | |
0.012 | - |
dehydroepiandrosterone | pH 5.5, 37°C | Rattus norvegicus | |
0.035 | - |
beta-estradiol | pH 5.5, 37°C | Rattus norvegicus | |
0.07 | - |
testosterone | pH 5.5, 37°C | Rattus norvegicus | |
0.29 | - |
beta-estradiol | pH 5.5, 37°C | Rattus norvegicus | |
1.2 | - |
Isoamylalcohol | pH 5.5, 37°C | Rattus norvegicus | |
1.7 | - |
1-amylalcohol | pH 5.5, 37°C | Rattus norvegicus | |
3 | - |
1-butanol | pH 5.5, 37°C | Rattus norvegicus | |
42 | - |
ethanol | pH 5.5, 37°C | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
6 * 28000, SDS-PAGE | Rattus norvegicus |
180000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + dehydroepiandrosterone | Rattus norvegicus | - |
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
rat | - |
Rattus norvegicus | - |
Sprague-Dawley, female | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.57 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-80°C, stable for several months | Rattus norvegicus |
4°C, loses 10% activity per week upon storage | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + 1-butanol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 1-butanoyl sulfate | - |
r | |
3'-phosphoadenylylsulfate + 11-deoxycorticosterone | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 11-deoxycorticosterone sulfate | - |
r | |
3'-phosphoadenylylsulfate + 2-propanol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 2-propyl sulfate | - |
r | |
3'-phosphoadenylylsulfate + aldosterone | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + aldosterone sulfate | - |
r | |
3'-phosphoadenylylsulfate + amyl alcohol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + amyl sulfate | - |
r | |
3'-phosphoadenylylsulfate + androst-5-en-3beta,17alpha-diol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
r | |
3'-phosphoadenylylsulfate + ascorbic acid | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
r | |
3'-phosphoadenylylsulfate + beta-estradiol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + beta-estradiol 3-O-sulfate | - |
r | |
3'-phosphoadenylylsulfate + chloramphenicol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
r | |
3'-phosphoadenylylsulfate + corticosterone | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + corticosterone sulfate | - |
r | |
3'-phosphoadenylylsulfate + cortisol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + cortisol sulfate | - |
r | |
3'-phosphoadenylylsulfate + D-propranolol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
r | |
3'-phosphoadenylylsulfate + dehydroepiandrosterone | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r | |
3'-phosphoadenylylsulfate + dehydroepiandrosterone | 3beta-hydroxyandrost-5-en-17-one | Rattus norvegicus | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r | |
3'-phosphoadenylylsulfate + ephedrine | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ephedrine sulfate | - |
r | |
3'-phosphoadenylylsulfate + ethanol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ethyl sulfate | - |
r | |
3'-phosphoadenylylsulfate + hydrocortisone | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + hydrocortisone sulfate | - |
r | |
3'-phosphoadenylylsulfate + isoamyl alcohol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + isoamyl sulfate | - |
r | |
3'-phosphoadenylylsulfate + L-propranolol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
r | |
3'-phosphoadenylylsulfate + ouabain | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
r | |
3'-phosphoadenylylsulfate + retinol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + retinol sulfate | - |
r | |
3'-phosphoadenylylsulfate + testosterone | 17beta-hydroxyandrost-4-en-3-one | Rattus norvegicus | adenosine 3',5'-bisphosphate + testosterone 3-sulfate | - |
r | |
additional information | does not utilize cholesterol, 2-naphthylamine, 2-naphthol, taurolithocholic acid, estrone, progesterone and N-hydroxy-2-acetylaminofluorene as sulfate acceptors | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 28000, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
dehydroepiandrosterone as substrate | Rattus norvegicus |
7.5 | - |
1-butanol as substrate | Rattus norvegicus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rattus norvegicus | isoelectric focusing, sucrose gradient in presence of 2.5% ampholines pH 5.0-7.0 | - |
5 |